PUBLICATION
Live imaging and modeling for shear stress quantification in the embryonic zebrafish heart
- Authors
- Boselli, F., Vermot, J.
- ID
- ZDB-PUB-150924-15
- Date
- 2016
- Source
- Methods (San Diego, Calif.) 94: 129-34 (Journal)
- Registered Authors
- Boselli, Francesco, Vermot, Julien
- Keywords
- Atherosclerosis, Embryonic heart, Heart dynamics, Hemodynamic harmonics, Method of fundamental solutions, Shear stress, Stokes flow, Zebrafish
- MeSH Terms
-
- Animals
- Biomechanical Phenomena
- Computer Simulation
- Embryo, Nonmammalian/physiology
- Heart/embryology
- Heart/physiology*
- Hemodynamics
- Hydrodynamics
- Microscopy, Confocal
- Models, Cardiovascular*
- Regional Blood Flow
- Zebrafish
- PubMed
- 26390811 Full text @ Methods
Citation
Boselli, F., Vermot, J. (2016) Live imaging and modeling for shear stress quantification in the embryonic zebrafish heart. Methods (San Diego, Calif.). 94:129-34.
Abstract
Hemodynamic shear stress is sensed by the endocardial cells composing the inner cell layer of the heart, and plays a major role in cardiac morphogenesis. Yet, the underlying hemodynamics and the associated mechanical stimuli experienced by endocardial cells remains poorly understood. Progress in the field has been hampered by the need for high temporal resolution imaging allowing the flow profiles generated in the beating heart to be resolved. To fill this gap, we propose a method to analyze the wall dynamics, the flow field, and the wall shear stress of the developing zebrafish heart. This method combines live confocal imaging and computational fluid dynamics to overcome difficulties related to live imaging of blood flow in the developing heart. To provide an example of the applicability of the method, we discuss the hemodynamic frequency content sensed by endocardial cells at the onset of valve formation, and how the fundamental frequency of the wall shear stress represents a unique mechanical cue to endocardial, heart-valve precursors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping