PUBLICATION
Characterization of F-spondin in Japanese flounder (Paralichthys olivaceus) and its role in the nervous system development of teleosts
- Authors
- Hu, H., Xin, N., Liu, J., Liu, M., Wang, Z., Wang, W., Zhang, Q., Qi, J.
- ID
- ZDB-PUB-150924-14
- Date
- 2016
- Source
- Gene 575(2 Pt 3): 623-31 (Journal)
- Registered Authors
- Keywords
- F-spondin, Japanese flounder, Nervous system development, Zebrafish
- MeSH Terms
-
- Animals
- Cloning, Molecular
- Extracellular Matrix Proteins/genetics*
- Extracellular Matrix Proteins/metabolism*
- Fish Proteins/genetics
- Fish Proteins/metabolism
- Flounder/anatomy & histology*
- Flounder/embryology
- Flounder/metabolism
- Gene Expression Regulation, Developmental
- Gonads/metabolism
- Nervous System/growth & development*
- Nervous System/metabolism
- Organ Specificity
- Phylogeny
- PubMed
- 26390814 Full text @ Gene
Citation
Hu, H., Xin, N., Liu, J., Liu, M., Wang, Z., Wang, W., Zhang, Q., Qi, J. (2016) Characterization of F-spondin in Japanese flounder (Paralichthys olivaceus) and its role in the nervous system development of teleosts. Gene. 575(2 Pt 3):623-31.
Abstract
F-spondin was originally isolated from the developing embryonic floor plate of vertebrates, secreting numerous kinds of neuron-related molecules. The protein performs a positive function in nervous system development, which is attributed to the high conservation of F-spondin protein, an extracellular matrix (ECM) protein in several species. However, its precise function remains unknown, especially in marine fish. In this study, the F-spondin of Japanese flounder (Paralichthys olivaceus). was cloned, and its expression pattern and structural characteristics were analyzed. The 2421bp-long cDNA ORF of PoF-spondin was obtained and divided into 14 exons spread over 61,496bp of the genomic sequence. Phylogenetic analysis showed that PoF-spondin was actually the ortholog of the human spon1 gene and shared high identities with other teleost spon1a genes. Quantitative RT-PCR analysis showed that PoF-spondin was maternally expressed, and transcripts were present from one-cell stage to hatching stage, peaking at tailbud stage. Tissue distribution analysis indicated that PoF-spondin was detectable mainly in the gonads (especially in the ovary) and the brain. Whole mount in situ hybridization analysis revealed that the PoF-spondin transcription distributed throughout the cleavage of the ball in the early stage and expressed at a high level in the floor plate of the trunk at tailbud and pre-hatching stages. Furthermore, the expression of genes related to nervous system development (spon1b, foxo3b, and foxj1a) was significantly increased after the injection of PoF-spondin into the embryos of wild-type zebrafish. Furthermore, PoF-spondin significantly suppressed the expression of the chordamesoderm marker gene ntl, increased the expression of otx2/krox20, ectoderm mark genes, and left the expression of dorsal mesodermal marker gene gsc unaffected at 50% epiboly stage in zebrafish. In short, our results suggest that PoF-spondin functions in the development of the teleost nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping