PUBLICATION
PEG-PLA Nanoparticles facilitate siRNA knockdown in adult zebrafish heart
- Authors
- Diao, J., Wang, H., Chang, N., Zhou, X.H., Zhu, X., Wang, J., Xiong, J.W.
- ID
- ZDB-PUB-150904-14
- Date
- 2015
- Source
- Developmental Biology 406(2): 196-202 (Journal)
- Registered Authors
- Xiong, Jing-Wei
- Keywords
- Heart regeneration, PEG-PLA nanoparticles, Sirna, Zebrafish
- MeSH Terms
-
- Animals
- Cell Proliferation/genetics
- Cell Proliferation/physiology
- Dual Specificity Phosphatase 6/genetics
- Gene Knockdown Techniques/methods*
- Isoenzymes/genetics
- Myocardium/cytology
- Myocardium/metabolism*
- Nanoparticles/metabolism*
- Polyethylene Glycols/metabolism*
- RNA Interference*
- RNA, Small Interfering/genetics
- RNA, Small Interfering/metabolism*
- Retinal Dehydrogenase/genetics
- Zebrafish/genetics*
- PubMed
- 26327645 Full text @ Dev. Biol.
Citation
Diao, J., Wang, H., Chang, N., Zhou, X.H., Zhu, X., Wang, J., Xiong, J.W. (2015) PEG-PLA Nanoparticles facilitate siRNA knockdown in adult zebrafish heart. Developmental Biology. 406(2):196-202.
Abstract
The remarkable regenerative capacity of the zebrafish has made it an important model organism for studying heart regeneration. However, current loss-of-function studies are limited by a lack of conditional-knockout and effective gene-knockdown methods for the adult heart. Here, we report a novel siRNA knockdown method facilitated by poly (ethylene glycol)-b-poly (D, l-lactide) (PEG-PLA) nanoparticles. The siRNA-encapsulated nanoparticles successfully entered cells and resulted in remarkable gene-specific knockdown in the adult heart. This effect was demonstrated by down-regulation of the Aldh1a2 and Dusp6 proteins after intrapleural delivery of nanoparticle-encapsulated siRNAs. Furthermore, siRNA-mediated knockdown of Aldh1a2 was sufficient to inhibit myocardial proliferation and decrease the numbers of Gata4-positive cardiomyocytes after ventricular resection. Therefore, the results of this work demonstrate that nanoparticle-facilitated siRNA delivery provides an alternative tool for loss-of-function studies of genes in the adult heart in particular and other organs in general in the adult zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping