|ZFIN ID: ZDB-PUB-150904-14|
PEG-PLA Nanoparticles facilitate siRNA knockdown in adult zebrafish heart
Diao, J., Wang, H., Chang, N., Zhou, X.H., Zhu, X., Wang, J., Xiong, J.W.
|Source:||Developmental Biology 406(2): 196-202 (Journal)|
|Registered Authors:||Xiong, Jing-Wei|
|Keywords:||Heart regeneration, PEG-PLA nanoparticles, Sirna, Zebrafish|
|PubMed:||26327645 Full text @ Dev. Biol.|
Diao, J., Wang, H., Chang, N., Zhou, X.H., Zhu, X., Wang, J., Xiong, J.W. (2015) PEG-PLA Nanoparticles facilitate siRNA knockdown in adult zebrafish heart. Developmental Biology. 406(2):196-202.
ABSTRACTThe remarkable regenerative capacity of the zebrafish has made it an important model organism for studying heart regeneration. However, current loss-of-function studies are limited by a lack of conditional-knockout and effective gene-knockdown methods for the adult heart. Here, we report a novel siRNA knockdown method facilitated by poly (ethylene glycol)-b-poly (D, l-lactide) (PEG-PLA) nanoparticles. The siRNA-encapsulated nanoparticles successfully entered cells and resulted in remarkable gene-specific knockdown in the adult heart. This effect was demonstrated by down-regulation of the Aldh1a2 and Dusp6 proteins after intrapleural delivery of nanoparticle-encapsulated siRNAs. Furthermore, siRNA-mediated knockdown of Aldh1a2 was sufficient to inhibit myocardial proliferation and decrease the numbers of Gata4-positive cardiomyocytes after ventricular resection. Therefore, the results of this work demonstrate that nanoparticle-facilitated siRNA delivery provides an alternative tool for loss-of-function studies of genes in the adult heart in particular and other organs in general in the adult zebrafish.