ZFIN ID: ZDB-PUB-150715-9
Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
Wehner, D., Jahn, C., Weidinger, G.
Date: 2015
Source: Journal of visualized experiments : JoVE   (100): e52756 (Journal)
Registered Authors: Jahn, Christopher, Wehner, Daniel, Weidinger, Gilbert
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Gene Expression
  • Regeneration/genetics
  • Regeneration/physiology*
  • Tail/physiology
  • Tetracycline/pharmacology*
  • Transcriptional Activation*
  • Zebrafish/genetics
  • Zebrafish/physiology*
PubMed: 26168286 Full text @ J. Vis. Exp.
The zebrafish has become a very important model organism for studying vertebrate development, physiology, disease, and tissue regeneration. A thorough understanding of the molecular and cellular mechanisms involved requires experimental tools that allow for inducible, tissue-specific manipulation of gene expression or signaling pathways. Therefore, we and others have recently adapted the TetON system for use in zebrafish. The TetON system facilitates temporally and spatially-controlled gene expression and we have recently used this tool to probe for tissue-specific functions of Wnt/beta-catenin signaling during zebrafish tail fin regeneration. Here we describe the workflow for using the TetON system to achieve inducible, tissue-specific gene expression in the adult regenerating zebrafish tail fin. This includes the generation of stable transgenic TetActivator and TetResponder lines, transgene induction and techniques for verification of tissue-specific gene expression in the fin regenerate. Thus, this protocol serves as blueprint for setting up a functional TetON system in zebrafish and its subsequent use, in particular for studying fin regeneration.