ZFIN ID: ZDB-PUB-150113-7
Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells
Qiao, J., Cui, S.J., Xu, L.L., Chen, S.J., Yao, J., Jiang, Y.H., Peng, G., Fang, C.Y., Yang, P.Y., Liu, F.
Date: 2015
Source: Oncotarget 6(2): 1171-89 (Journal)
Registered Authors: Chen, Sijie, Liu, Feng, Peng, Gang
Keywords: none
MeSH Terms: Adult; Aged; Aged, 80 and over; Cell Line, Tumor; Cell Movement/genetics (all 27) expand
PubMed: 25577646 Full text @ Oncotarget
ABSTRACT
Gastric cancer (GC) is the fourth and fifth most common cancer in men and women, respectively. We identified 2,750 proteins at false discovery rates of 1.3% (protein) and 0.03% (spectrum) by comparing the proteomic profiles of three GC and a normal gastric cell lines. Nine proteins were significantly dysregulated in all three GC cell lines, including filamin C, a muscle-specific filamin and a large actin-cross-linking protein. Downregulation of filamin C in GC cell lines and tissues were verified using quantitative PCR and immunohistochemistry. Data-mining using public microarray datasets shown that filamin C was significantly reduced in many human primary and metastasis cancers. Transient expression or silencing of filamin C affected the proliferation and colony formation of cancer cells. Silencing of endogenous filamin C enhanced cancer cell migration and invasion, whereas ectopic expression of filamin C had opposing effects. Silencing of filamin C increased the expression of matrix metallopeptidase 2 and improved the metastasis of prostate cancer in a zebrafish model. High filamin C associated with better prognosis of prostate cancer, leukemia and breast cancer patients. These findings establish a functional role of filamin C in human cancers and these data will be valuable for further study of its mechanisms.
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