PUBLICATION
A Defined Zebrafish Line for High-Throughput Genetics and Genomics: NHGRI-1
- Authors
- LaFave, M.C., Varshney, G.K., Vemulapalli, M., Mullikin, J.C., Burgess, S.M.
- ID
- ZDB-PUB-140712-18
- Date
- 2014
- Source
- Genetics 198(1): 167-70 (Journal)
- Registered Authors
- Burgess, Shawn, Varshney, Gaurav
- Keywords
- CRISPR, SNV, genome sequence, variants, zebrafish
- MeSH Terms
-
- Animals
- Animals, Inbred Strains
- Genomics/methods*
- High-Throughput Screening Assays/methods*
- Zebrafish/genetics*
- PubMed
- 25009150 Full text @ Genetics
Citation
LaFave, M.C., Varshney, G.K., Vemulapalli, M., Mullikin, J.C., Burgess, S.M. (2014) A Defined Zebrafish Line for High-Throughput Genetics and Genomics: NHGRI-1. Genetics. 198(1):167-70.
Abstract
Substantial intra-strain variation at the nucleotide level complicates molecular and genetic studies in zebrafish, such as the use of CRISPRs or morpholinos to inactivate genes. In the absence of robust inbred zebrafish lines, we generated NHGRI-1, a healthy and fecund strain derived from founder parents we sequenced to a depth of ~50x. Within this strain, we have identified the majority of the genome that matches the reference sequence, and documented most of the variants. This strain has utility for many reasons, but in particular it will be useful for any researcher who needs to know the exact sequence (with all variants) of a particular genomic region, or who wants to be able to robustly map sequences back to a genome with all possible variants defined.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping