PUBLICATION

Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting

Authors
Astin, J.W., Haggerty, M.J., Okuda, K.S., Le Guen, L., Misa, J.P., Tromp, A., Hogan, B.M., Crosier, K.E., Crosier, P.S.
ID
ZDB-PUB-140607-9
Date
2014
Source
Development (Cambridge, England)   141(13): 2680-90 (Journal)
Registered Authors
Crosier, Phil, Hogan, Ben M., Le Guen, Ludovic, Okuda, Kazuhide Shaun
Keywords
none
MeSH Terms
  • Animals
  • Calcium-Binding Proteins/metabolism
  • DNA Primers/genetics
  • In Situ Hybridization
  • Lymphangiogenesis/genetics
  • Lymphangiogenesis/physiology*
  • Microscopy, Confocal
  • Morpholinos/genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Statistics, Nonparametric
  • Vascular Endothelial Growth Factor C/deficiency*
  • Vascular Endothelial Growth Factor D/metabolism*
  • Zebrafish/embryology*
  • Zebrafish Proteins/deficiency*
  • Zebrafish Proteins/metabolism*
PubMed
24903752 Full text @ Development
Abstract
Lymphangiogenesis is a dynamic process that involves the sprouting of lymphatic endothelial cells (LECs) from veins to form lymphatic vessels. Vegfr3 signalling, through its ligand Vegfc and the extracellular protein Ccbe1, is essential for the sprouting of LECs to form the trunk lymphatic network. In this study we determined whether Vegfr3, Vegfc and Ccbe1 are also required for development of the facial and intestinal lymphatic networks in the zebrafish embryo. Whereas Vegfr3 and Ccbe1 are required for the development of all lymphatic vessels, Vegfc is dispensable for facial lymphatic sprouting but not for the complete development of the facial lymphatic network. We show that zebrafish vegfd is expressed in the head, genetically interacts with ccbe1 and can rescue the lymphatic defects observed following the loss of vegfc. Finally, whereas knockdown of vegfd has no phenotype, double knockdown of both vegfc and vegfd is required to prevent facial lymphatic sprouting, suggesting that Vegfc is not essential for all lymphatic sprouting and that Vegfd can compensate for loss of Vegfc during lymphatic development in the zebrafish head.
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Human Disease / Model
Sequence Targeting Reagents
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Antibodies
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