|ZFIN ID: ZDB-PUB-140513-14|
Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism
Miyares, R.L., de Rezende, V.B., Farber, S.A.
|Source:||Disease models & mechanisms 7(7): 915-27 (Journal)|
|Registered Authors:||Farber, Steven, Miyares, Rosa|
|PubMed:||24812437 Full text @ Dis. Model. Mech.|
Miyares, R.L., de Rezende, V.B., Farber, S.A. (2014) Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism. Disease models & mechanisms. 7(7):915-27.
ABSTRACTDyslipidemias are a major cause of morbidity and mortality in the world, particularly in developed nations. Investigating lipid and lipoprotein metabolism in experimentally tractable animal models is a critical step toward understanding and treating human dyslipidemias. The zebrafish, a well-established embryological model, is emerging as a notable system for studies in lipid metabolism. Here, we describe the value of the lecithotrophic, or yolk metabolizing, stages of the zebrafish as a model for studying lipid metabolism and lipoprotein transport. We demonstrate methods to assay yolk lipid metabolism in embryonic and larval zebrafish. These methods promote efficient uptake of yolk-delivered labeled fatty acids into the circulation. Using a genetic model for abetalipoproteinemia, we show that uptake of labeled fatty acids into the circulation is dependent on lipoprotein production. Further, we examine the metabolic fate of exogenously delivered fatty acids by assaying their incorporation into complex lipids. Moreover, we demonstrate that this technique is amenable to genetic and pharmacologic studies.