ZFIN ID: ZDB-PUB-140513-14
Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism
Miyares, R.L., de Rezende, V.B., Farber, S.A.
Date: 2014
Source: Disease models & mechanisms   7(7): 915-27 (Journal)
Registered Authors: Farber, Steven, Miyares, Rosa
Keywords: none
MeSH Terms:
  • Absorption, Physiological/drug effects
  • Animals
  • Biological Assay/methods*
  • Biological Transport/drug effects
  • Boron Compounds/metabolism
  • Egg Yolk/drug effects
  • Egg Yolk/metabolism*
  • Embryonic Development/drug effects
  • Enzyme Inhibitors/pharmacology
  • Fatty Acids/metabolism
  • Humans
  • Larva/drug effects
  • Larva/metabolism
  • Lipid Metabolism*/drug effects
  • Lipoproteins, LDL/metabolism
  • Reproducibility of Results
  • Staining and Labeling
  • Time Factors
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism
PubMed: 24812437 Full text @ Dis. Model. Mech.
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ABSTRACT
Dyslipidemias are a major cause of morbidity and mortality in the world, particularly in developed nations. Investigating lipid and lipoprotein metabolism in experimentally tractable animal models is a critical step toward understanding and treating human dyslipidemias. The zebrafish, a well-established embryological model, is emerging as a notable system for studies in lipid metabolism. Here, we describe the value of the lecithotrophic, or yolk metabolizing, stages of the zebrafish as a model for studying lipid metabolism and lipoprotein transport. We demonstrate methods to assay yolk lipid metabolism in embryonic and larval zebrafish. These methods promote efficient uptake of yolk-delivered labeled fatty acids into the circulation. Using a genetic model for abetalipoproteinemia, we show that uptake of labeled fatty acids into the circulation is dependent on lipoprotein production. Further, we examine the metabolic fate of exogenously delivered fatty acids by assaying their incorporation into complex lipids. Moreover, we demonstrate that this technique is amenable to genetic and pharmacologic studies.
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