ZFIN ID: ZDB-PUB-140303-35
Msd1/SSX2IP-dependent microtubule anchorage ensures spindle orientation and primary cilia formation
Hori, A., Ikebe, C., Tada, M., and Toda, T.
Date: 2014
Source: EMBO reports   15(2): 175-184 (Journal)
Registered Authors: Tada, Masazumi
Keywords: none
MeSH Terms:
  • Animals
  • Cilia/metabolism
  • HeLa Cells
  • Humans
  • Microtubule-Associated Proteins/genetics
  • Microtubule-Associated Proteins/metabolism*
  • Microtubules/metabolism*
  • Neoplasm Proteins/genetics
  • Neoplasm Proteins/metabolism*
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism*
  • Spindle Apparatus/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 24397932 Full text @ EMBO Rep.

Anchoring microtubules to the centrosome is critical for cell geometry and polarity, yet the molecular mechanism remains unknown. Here we show that the conserved human Msd1/SSX2IP is required for microtubule anchoring. hMsd1/SSX2IP is delivered to the centrosome in a centriolar satellite-dependent manner and binds the microtubule-nucleator γ-tubulin complex. hMsd1/SSX2IP depletion leads to disorganised interphase microtubules and misoriented mitotic spindles with reduced length and intensity. Furthermore, hMsd1/SSX2IP is essential for ciliogenesis, and during zebrafish embryogenesis, knockdown of its orthologue results in ciliary defects and disturbs left-right asymmetry. We propose that the Msd1 family comprises conserved microtubule-anchoring proteins.