PUBLICATION
Fish in chips: an automated microfluidic device to study drug dynamics in vivo using zebrafish embryos
- Authors
- Zheng, C., Zhou, H., Liu, X., Pang, Y., Zhang, B., and Huang, Y.
- ID
- ZDB-PUB-140127-5
- Date
- 2014
- Source
- Chemical communications (Cambridge, England) 50(8): 981-984 (Journal)
- Registered Authors
- Liu, Xinxing, Zhang, Bo
- Keywords
- none
- MeSH Terms
-
- Zebrafish/growth & development*
- Fish Proteins/metabolism
- Hedgehog Proteins/metabolism
- Veratrum Alkaloids/chemistry
- Veratrum Alkaloids/toxicity*
- Animals
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Signal Transduction/drug effects
- Automation
- Embryonic Development/drug effects
- Animals, Genetically Modified/growth & development
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Microfluidic Analytical Techniques/instrumentation
- Microfluidic Analytical Techniques/methods*
- PubMed
- 24305733 Full text @ Chem. Commun. (Camb.)
Citation
Zheng, C., Zhou, H., Liu, X., Pang, Y., Zhang, B., and Huang, Y. (2014) Fish in chips: an automated microfluidic device to study drug dynamics in vivo using zebrafish embryos. Chemical communications (Cambridge, England). 50(8):981-984.
Abstract
Interference of the Hedgehog (Hh) signaling pathway by cyclopamine leads to abnormal embryonic development. We monitor this dynamical drug effect in zebrafish embryos with highly precise microenvironment control using an integrated microfluidic device. This chip-based platform, which is programmable and automated, greatly facilitates the accuracy and reproducibility of the in vivo assays.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping