PUBLICATION

Construction and application of site-specific artificial nucleases for targeted gene editing

Authors
Kok, F.O., Gupta, A., Lawson, N.D., and Wolfe, S.A.
ID
ZDB-PUB-140101-15
Date
2014
Source
Methods in molecular biology (Clifton, N.J.)   1101: 267-303 (Chapter)
Registered Authors
Gupta, Ankit, Lawson, Nathan, Wolfe, Scot A.
Keywords
zinc finger nucleases, ZFNs, TALE nucleases, TALENs, zebrafish, modular assembly, golden gate assembly
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • DNA Primers/genetics
  • Deoxyribonucleases/genetics*
  • Escherichia coli
  • Gene Targeting/methods*
  • INDEL Mutation*
  • Molecular Sequence Data
  • Protein Engineering/methods*
  • Substrate Specificity
  • Zebrafish/genetics*
  • Zinc Fingers
PubMed
24233786 Full text @ Meth. Mol. Biol.
Abstract

Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping