ZFIN ID: ZDB-PUB-140101-15
Construction and application of site-specific artificial nucleases for targeted gene editing
Kok, F.O., Gupta, A., Lawson, N.D., and Wolfe, S.A.
Date: 2014
Source: Methods in molecular biology (Clifton, N.J.)   1101: 267-303 (Chapter)
Registered Authors: Gupta, Ankit, Lawson, Nathan, Wolfe, Scot A.
Keywords: zinc finger nucleases, ZFNs, TALE nucleases, TALENs, zebrafish, modular assembly, golden gate assembly
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • DNA Primers/genetics
  • Deoxyribonucleases/genetics*
  • Escherichia coli
  • Gene Targeting/methods*
  • INDEL Mutation*
  • Molecular Sequence Data
  • Protein Engineering/methods*
  • Substrate Specificity
  • Zebrafish/genetics*
  • Zinc Fingers
PubMed: 24233786 Full text @ Meth. Mol. Biol.

Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.