ZFIN Person: Wolfe, Scot A.

ZFIN ID: ZDB-PERS-080806-1

Wolfe, Scot A.

Email:	scot.wolfe@umassmed.edu
URL:	http://labs.umassmed.edu/WolfeLab
Affiliation:	Wolfe Laboratory
Address:	Program in Gene Function & Expression Department of Biochemistry & Molecular Pharmacology UMass Medical School 6th Floor, Lazare Research Building 364 Plantation St. Worcester, MA 01605-2324 USA
Country:	United States
Phone:	(508) 856 3953
Fax:	(508) 856 5460
ORCID ID:

BIOGRAPHY AND RESEARCH INTERESTS

PUBLICATIONS

Shin, M., Yin, H.M., Shih, Y.H., Nozaki, T., Portman, D., Toles, B., Kolb, A., Luk, K., Isogai, S., Ishida, K., Hanasaka, T., Parsons, M.J., Wolfe, S.A., Burns, C.E., Burns, C.G., Lawson, N.D. (2023) Generation and application of endogenously floxed alleles for cell-specific knockout in zebrafish. Developmental Cell. 58(22):2614-2626.e7

Ponnienselvan, K., Liu, P., Nyalile, T., Oikemus, S., Maitland, S.A., Lawson, N.D., Luban, J., Wolfe, S.A. (2023) Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiency. Nucleic acids research. 51(13):6966-6980

Shin, M., Nozaki, T., Idrizi, F., Isogai, S., Ogasawara, K., Ishida, K., Yuge, S., Roscoe, B., Wolfe, S.A., Fukuhara, S., Mochizuki, N., Deguchi, T., Lawson, N.D. (2019) Valves Are a Conserved Feature of the Zebrafish Lymphatic System. Developmental Cell. 51(3):374-386.e5

Liu, P., Luk, K., Shin, M., Idrizi, F., Kwok, S., Roscoe, B., Mintzer, E., Suresh, S., Morrison, K., Frazão, J.B., Bolukbasi, M.F., Ponnienselvan, K., Luban, J., Zhu, L.J., Lawson, N.D., Wolfe, S.A. (2019) Enhanced Cas12a editing in mammalian cells and zebrafish. Nucleic acids research. 47(8):4169-4180

Kok, F.O., Shin, M., Ni, C., Gupta, A., Grosse, A.S., van Impel, A., Kirchmaier, B.C., Peterson-Maduro, J., Kourkoulis, G., Male, I., DeSantis, D.F., Sheppard-Tindell, S., Ebarasi, L., Betsholtz, C., Schulte-Merker, S., Wolfe, S.A., Lawson, N.D. (2015) Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish. Developmental Cell. 32(1):97-108

Weicksel, S.E., Gupta, A., Zannino, D.A., Wolfe, S.A., Sagerström, C.G. (2014) Targeted germ line disruptions reveal general and species-specific roles for paralog group 1 hox genes in zebrafish. BMC Developmental Biology. 14:25

Kok, F.O., Gupta, A., Lawson, N.D., and Wolfe, S.A. (2014) Construction and application of site-specific artificial nucleases for targeted gene editing. Methods in molecular biology (Clifton, N.J.). 1101:267-303

Gupta, A., Hall, V.L., Kok, F.O., Shin, M., McNulty, J.C., Lawson, N.D., and Wolfe, S.A. (2013) Targeted Chromosomal Deletions and Inversions in Zebrafish. Genome research. 23(6):1008-17

Shin, J., Padmanabhan, A., de Groh, E.D., Lee, J.S., Haidar, S., Dahlberg, S., Guo, F., He, S., Wolman, M.A., Granato, M., Lawson, N.D., Wolfe, S.A., Kim, S.H., Solnica-Krezel, L., Kanki, J.P., Ligon, K.L., Epstein, J.A., and Look, A.T. (2012) Zebrafish neurofibromatosis type 1 genes have redundant functions in tumorigenesis and embryonic development. Disease models & mechanisms. 5(6):881-894

Gupta, A., Christensen, R.G., Rayla, A.L., Lakshmanan, A., Stormo, G.D., and Wolfe, S.A. (2012) An optimized two-finger archive for ZFN-mediated gene targeting. Nature Methods. 9(6):588-590

Zhu, C., Smith, T., McNulty, J., Rayla, A.L., Lakshmanan, A., Siekmann, A.F., Buffardi, M., Meng, X., Shin, J., Padmanabhan, A., Cifuentes, D., Giraldez, A.J., Look, A.T., Epstein, J.A., Lawson, N.D., and Wolfe, S.A. (2011) Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development (Cambridge, England). 138(20):4555-4564

Lawson, N.D., and Wolfe, S.A. (2011) Forward and reverse genetic approaches for the analysis of vertebrate development in the zebrafish. Developmental Cell. 21(1):48-64

Bussmann, J., Wolfe, S.A., and Siekmann, A.F. (2011) Arterial-venous network formation during brain vascularization involves hemodynamic regulation of chemokine signaling. Development (Cambridge, England). 138(9):1717-1726

Gupta, A., Meng, X., Zhu, L.J., Lawson, N.D., and Wolfe, S.A. (2011) Zinc finger protein-dependent and -independent contributions to the in vivo off-target activity of zinc finger nucleases. Nucleic acids research. 39(1):381-392

Cifuentes, D., Xue, H., Taylor, D.W., Patnode, H., Mishima, Y., Cheloufim, S., Ma, E., Mane, S., Hannon, G.J., Lawson, N.D., Wolfe, S.A., and Giraldez, A.J. (2010) A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity. Science (New York, N.Y.). 328(5986):1694-1698

Siekmann, A.F., Standley, C., Fogarty, K.E., Wolfe, S.A., and Lawson, N.D. (2009) Chemokine signaling guides regional patterning of the first embryonic artery. Genes & Development. 23(19):2272-2277

Meng, X., Noyes, M.B., Zhu, L.J., Lawson, N.D., and Wolfe, S.A. (2008) Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat. Biotechnol.. 26(6):695-701

NON-ZEBRAFISH PUBLICATIONS