PUBLICATION

Mutagenesis and phenotyping resources in zebrafish for studying development and human disease

Authors
Varshney, G.K., and Burgess, S.M.
ID
ZDB-PUB-131121-23
Date
2014
Source
Briefings in Functional Genomics   13(2): 82-94 (Review)
Registered Authors
Burgess, Shawn
Keywords
zebrafish, mutagenesis, phenotyping, resources, knockouts
MeSH Terms
  • Animals
  • Disease/genetics*
  • Disease Models, Animal
  • Genomics/methods*
  • Humans
  • Mutagenesis/genetics*
  • Phenotype
  • Zebrafish/genetics*
PubMed
24162064 Full text @ Brief. Funct. Genomics
Abstract

The zebrafish (Danio rerio) is an important model organism for studying development and human disease. The zebrafish has an excellent reference genome and the functions of hundreds of genes have been tested using both forward and reverse genetic approaches. Recent years have seen an increasing number of large-scale mutagenesis projects and the number of mutants or gene knockouts in zebrafish has increased rapidly, including for the first time conditional knockout technologies. In addition, targeted mutagenesis techniques such as zinc finger nucleases, transcription activator-like effector nucleases and clustered regularly interspaced short sequences (CRISPR) or CRISPR-associated (Cas), have all been shown to effectively target zebrafish genes as well as the first reported germline homologous recombination, further expanding the utility and power of zebrafish genetics. Given this explosion of mutagenesis resources, it is now possible to perform systematic, high-throughput phenotype analysis of all zebrafish gene knockouts.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping