PUBLICATION

Blood Flow Changes Coincide with Cellular Rearrangements during Blood Vessel Pruning in Zebrafish Embryos

Authors
Kochhan, E., Lenard, A., Ellertsdottir, E., Herwig, L., Affolter, M., Belting, H.G., and Siekmann, A.F.
ID
ZDB-PUB-131119-41
Date
2013
Source
PLoS One   8(10): e75060 (Journal)
Registered Authors
Affolter, Markus, Belting, Heinz-Georg Paul (Henry), Ellertsdottir, Elin, Siekmann, Arndt Friedrich
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Blood Vessels/cytology*
  • Blood Vessels/embryology
  • Cell Death
  • Embryo, Nonmammalian
  • Endothelial Cells/cytology*
  • Endothelium, Vascular/cytology*
  • Endothelium, Vascular/embryology
  • Eye/blood supply*
  • Eye/cytology
  • Eye/embryology
  • Hemodynamics/physiology*
  • Morphogenesis
  • Neovascularization, Physiologic
  • Time-Lapse Imaging
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
24146748 Full text @ PLoS One
Abstract

After the initial formation of a highly branched vascular plexus, blood vessel pruning generates a hierarchically structured network with improved flow characteristics. We report here on the cellular events that occur during the pruning of a defined blood vessel in the eye of developing zebrafish embryos. Time-lapse imaging reveals that the connection of a new blood vessel sprout with a previously perfused multicellular endothelial tube leads to the formation of a branched, Y-shaped structure. Subsequently, endothelial cells in parts of the previously perfused branch rearrange from a multicellular into a unicellular tube, followed by blood vessel detachment. This process is accompanied by endothelial cell death. Finally, we show that differences in blood flow between neighboring vessels are important for the completion of the pruning process. Our data suggest that flow induced changes in tubular architecture ensure proper blood vessel pruning.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes