ZFIN ID: ZDB-PUB-131113-5
p35 promotes the differentiation of amacrine cell subtype in the zebrafish retina under the regulation of egr1
Zhang, L., Bonilla, S., Zhang, Y., and Leung, Y.F.
Date: 2014
Source: Developmental dynamics : an official publication of the American Association of Anatomists   243(2): 315-23 (Journal)
Registered Authors: Bonilla, Sylvia, Leung, Yuk Fai, Zhang, Liyun
Keywords: p35, cdk5r1b, zebrafish, retinal development, cdk5, egr1, amacrine cells, smarca4
MeSH Terms:
  • Amacrine Cells/physiology*
  • Animals
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology*
  • DNA Primers/genetics
  • Early Growth Response Protein 1/metabolism*
  • Gene Expression Regulation, Developmental/genetics*
  • Gene Knockdown Techniques
  • Image Processing, Computer-Assisted
  • Immunohistochemistry
  • In Situ Hybridization
  • Microscopy, Fluorescence
  • Morpholinos/genetics
  • Nerve Tissue Proteins/metabolism*
  • Parvalbumins/metabolism
  • Retina/embryology*
  • Retina/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/embryology*
PubMed: 24115595 Full text @ Dev. Dyn.

Background: Early growth response 1 (egr1) is a transcription factor (TF) for controlling the differentiation of Parvalbumin (Parv) -expressing amacrine cells (ACs) in zebrafish. However, the downstream factors of this process have not been identified. The purpose of this study was to investigate the role of p35, a neuronal-specific activator of cyclin-dependent kinase 5 (Cdk5) and a known in vitro target of egr1, in the differentiation of these ACs. Results: In the p35-knockdown retinas, Parv+ but not islet1+ ACs were specifically reduced. This phenotype was highly similar to that in the Egr1-knockdown retinas. Furthermore, p35 expression was reduced in the Egr1-knockdown retinas, particularly in the AC region; while egr1 was only modestly reduced in this region in the p35-knockdown retinas. Conclusions: p35 likely acts downstream of egr1 to control the differentiation of Parv+ ACs.