ZFIN ID: ZDB-PUB-130903-27
Tanshinone IIA exhibits anticonvulsant activity in zebrafish and mouse seizure models
Buenafe, O.E., Orellana Paucar, A.M., Maes, J., Huang, H., De Borggraeve, W.M., Crawford, A.D., Luyten, W., Esguerra, C.V., and de Witte, P.
Date: 2013
Source: ACS Chemical Neuroscience   4(11): 1479-87 (Journal)
Registered Authors: Crawford, Alexander, Esguerra, Camila V.
Keywords: none
MeSH Terms:
  • Abietanes/administration & dosage*
  • Abietanes/physiology
  • Abietanes/therapeutic use
  • Alzheimer Disease/drug therapy
  • Animals
  • Anticonvulsants/administration & dosage*
  • Anticonvulsants/therapeutic use
  • Brain Ischemia/drug therapy
  • Disease Models, Animal
  • Disease Progression
  • Drugs, Chinese Herbal/therapeutic use*
  • Fertilization in Vitro/drug effects
  • Injections, Intraventricular
  • Larva/drug effects
  • Male
  • Medicine, Chinese Traditional/methods*
  • Mice
  • Microinjections
  • Neuroprotective Agents/administration & dosage
  • Neuroprotective Agents/therapeutic use
  • Pentylenetetrazole/administration & dosage
  • Pentylenetetrazole/antagonists & inhibitors*
  • Pentylenetetrazole/toxicity
  • Plant Extracts/administration & dosage
  • Plant Extracts/therapeutic use
  • Plant Roots/chemistry
  • Proto-Oncogene Proteins c-fos/antagonists & inhibitors
  • Proto-Oncogene Proteins c-fos/biosynthesis
  • Salvia miltiorrhiza/chemistry
  • Seizures/diagnosis
  • Seizures/drug therapy*
  • Seizures/mortality
  • Small Molecule Libraries/administration & dosage
  • Small Molecule Libraries/therapeutic use
  • Zebrafish/embryology
PubMed: 23937066 Full text @ ACS Chem. Neurosci.
ABSTRACT

Danshen or Chinese red sage (Salvia miltiorrhiza, Bunge) is used by traditional Chinese medicine (TCM) practitioners to treat neurological, cardio- and cerebrovascular disorders, and is included in TCM formulations to control epileptic seizures. In this study, acetonic crude extracts of danshen inhibited pentylenetetrazol (PTZ)-induced seizure activity in zebrafish larvae. Subsequent zebrafish bioassay-guided fractionation of the extract resulted in the isolation of four major tanshinones present which suppressed PTZ-induced activity to varying degrees. One of the active tanshinones, tanshinone IIA, also reduced c-fos expression in the brains of PTZ-exposed zebrafish larvae. In rodent seizure models, tanshinone IIA showed anticonvulsive activity in the mouse 6-Hz psychomotor seizure test in a bi-phasic manner, and modifies seizure thresholds in a complex manner for the mouse i.v. PTZ seizure assay. Interestingly, tanshinone IIA is used as a prescription drug in China to address cerebral ischemia in patients. Here, we provide the first in vivo evidence demonstrating that tanshinone IIA has anticonvulsant properties as well.

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