PUBLICATION

Toxicogenomic Responses of Zebrafish Embryos/Larvae to Tris(1,3-dichloro-2-propyl) Phosphate (TDCPP) Reveal Possible Molecular Mechanisms of Developmental Toxicity

Authors
Fu, J., Han, J., Zhou, B., Gong, Z., Santos, E.M., Huo, X., Zheng, W., Liu, H., Yu, H., and Liu, C.
ID
ZDB-PUB-130830-5
Date
2013
Source
Environmental science & technology   47(18): 10574-82 (Journal)
Registered Authors
Gong, Zhiyuan, Santos, Eduarda
Keywords
none
MeSH Terms
  • Animals
  • Bone and Bones/abnormalities
  • Cartilage/abnormalities
  • Embryo, Nonmammalian/abnormalities
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development/drug effects
  • Flame Retardants/pharmacokinetics
  • Flame Retardants/toxicity*
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental/drug effects*
  • Muscle, Skeletal/abnormalities
  • Oligonucleotide Array Sequence Analysis
  • Organophosphates/pharmacokinetics
  • Organophosphates/toxicity*
  • Zebrafish
PubMed
23919627 Full text @ Env. Sci. Tech.
CTD
23919627
Abstract

Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is frequently present in indoor dust and can be detected in human milk. In order to evaluate the effects of TDCPP on vertebrate development, zebrafish embryos/larvae were used as an animal model to examine developmental phenotypes and explore possible mechanisms of toxicity by employing microarrays and iTRAQ labeling quantitative proteomics. The results demonstrated that treatment with TDCPP (3 μM) from 0.75 h postfertilization (hpf) inhibited cell rearrangement at 4 hpf, caused delay in epiboly at 5.7 and 8.5 hpf, and led to abnormal development (e.g., short tail, reduced body size) and lethality between 14 and 45 hpf, which might be related with altered expression of genes regulating embryogenesis. Furthermore, trunk curvature was observed as the main phenotype in 96 hpf zebrafish larvae exposed to 1 or 3 μM TDCPP, possibly by changing somite formation and expression of proteins related to fast muscle and cartilage development. Collectively, our results suggest that exposure to TDCPP causes developmental toxicity in vertebrates and warrant the need for studies to evaluate the potential health risks of TDCPP to developing human embryos/infants/children, due to its frequent presence in indoor dust and potential for human exposure.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping