ZFIN ID: ZDB-PUB-130211-4
Efficient genome editing in zebrafish using a CRISPR-Cas system
Hwang, W.Y., Fu, Y., Reyon, D., Maeder, M.L., Tsai, S.Q., Sander, J.D., Peterson, R.T., Yeh, J.R., and Joung, J.K.
Date: 2013
Source: Nat. Biotechnol. 31(3): 227-229 (Journal)
Registered Authors: Peterson, Randall, Yeh, Jing-Ruey (Joanna)
Keywords: none
MeSH Terms:
  • Animals
  • Base Sequence
  • DNA/genetics
  • DNA Cleavage
  • Embryo, Nonmammalian
  • Endonucleases/genetics
  • Genetic Engineering
  • Genome*
  • Inverted Repeat Sequences*
  • Molecular Sequence Data
  • Mutation
  • Nucleic Acid Conformation
  • RNA Editing
  • RNA, Guide/genetics*
  • Zebrafish/genetics*
PubMed: 23360964 Full text @ Nat. Biotechnol.
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ABSTRACT

In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator–like effector nucleases.

ADDITIONAL INFORMATIONNo data available