PUBLICATION

HyPer-3: a genetically encoded H2O2 probe with improved performance for ratiometric and fluorescence lifetime imaging

Authors
Bilan, D.S., Pase, L., Joosen, L., Gorokhovatsky, A.Y., Ermakova, Y.G., Gadella, T.W., Grabher, C., Schultz, C., Lukyanov, S., and Belousov, V.V.
ID
ZDB-PUB-121227-3
Date
2013
Source
ACS Chemical Biology   8(3): 535-542 (Journal)
Registered Authors
Grabher, Clemens, Pase, Luke
Keywords
none
MeSH Terms
  • Animals
  • Fluorescence*
  • Fluorescent Dyes/analysis*
  • Hydrogen Peroxide/analysis*
  • Hydrogen Peroxide/metabolism
  • Larva/metabolism
  • Luminescent Measurements/methods*
  • Oxidation-Reduction
  • Zebrafish/growth & development
  • Zebrafish/metabolism
PubMed
23256573 Full text @ ACS Chem. Biol.
Abstract

High performance sensors for reactive oxygen species are instrumental to monitor dynamic events in cells and organisms. Here we present HyPer-3, a genetically encoded fluorescent indicator for intracellular H2O2 exhibiting improved performance with respect to response time and speed. HyPer-3 has an expanded dynamic range compared to HyPer and significantly faster oxidation/reduction dynamics compared to HyPer-2. We demonstrate this performance by in vivo imaging of tissue-scale H2O2 gradients in zebrafish larvae. Moreover, HyPer-3 was successfully employed for single-wavelength fluorescent lifetime imaging of H2O2 levels both in vitro and in vivo.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping