ZFIN ID: ZDB-PUB-121227-3
HyPer-3: a genetically encoded H2O2 probe with improved performance for ratiometric and fluorescence lifetime imaging
Bilan, D.S., Pase, L., Joosen, L., Gorokhovatsky, A.Y., Ermakova, Y.G., Gadella, T.W., Grabher, C., Schultz, C., Lukyanov, S., and Belousov, V.V.
Date: 2013
Source: ACS Chemical Biology 8(3): 535-542 (Journal)
Registered Authors: Grabher, Clemens, Pase, Luke
Keywords: none
MeSH Terms:
  • Animals
  • Fluorescence*
  • Fluorescent Dyes/analysis*
  • Hydrogen Peroxide/analysis*
  • Hydrogen Peroxide/metabolism
  • Larva/metabolism
  • Luminescent Measurements/methods*
  • Oxidation-Reduction
  • Zebrafish/growth & development
  • Zebrafish/metabolism
PubMed: 23256573 Full text @ ACS Chem. Biol.
ABSTRACT

High performance sensors for reactive oxygen species are instrumental to monitor dynamic events in cells and organisms. Here we present HyPer-3, a genetically encoded fluorescent indicator for intracellular H2O2 exhibiting improved performance with respect to response time and speed. HyPer-3 has an expanded dynamic range compared to HyPer and significantly faster oxidation/reduction dynamics compared to HyPer-2. We demonstrate this performance by in vivo imaging of tissue-scale H2O2 gradients in zebrafish larvae. Moreover, HyPer-3 was successfully employed for single-wavelength fluorescent lifetime imaging of H2O2 levels both in vitro and in vivo.

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