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Research
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ZIRC
ZFIN ID: ZDB-PERS-000301-2
Grabher, Clemens
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BIOGRAPHY AND RESEARCH INTERESTS
EDUCATION AND EXPERIENCE

11er Nahrungsmittel GmbH, Frastanz, Austria
CEO, 2015-present

Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany
Groupleader, 2009-2015
Innate Immunity

Dana-Farber Cancer Institute (DFCI) and Harvard Medical School (HMS), Boston, USA
Laboratory of Dr. A. Thomas Look
Postdoctoral Fellow, 2004-2008
Innate Immunity; miRNA Function in Hematopoiesis; Modeling of Acute Myeloid Leukemia and Myelodisplastic Syndrome in Zebrafish

European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
Laboratory of Dr. J. Wittbrodt
PhD Student and Postdoctoral Fellow, 1999-2004
Innate Immunity; Novel Approaches to Transgenesis in Teleost Fish (Medaka and Zebrafish)

Vienna Biocenter, University of Vienna, Vienna, Austria
Laboratory of Dr. T. Decker
Diploma Student, 1996-1998
Analysis of STAT5 in Myelopoiesis


PUBLICATIONS
Middel, V., Zhou, L., Takamiya, M., Beil, T., Shahid, M., Roostalu, U., Grabher, C., Rastegar, S., Reischl, M., Nienhaus, G.U., Strähle, U. (2016) Dysferlin-mediated phosphatidylserine sorting engages macrophages in sarcolemma repair. Nature communications. 7:12875
Takamiya, M., Xu, F., Suhonen, H., Gourain, V., Yang, L., Ho, N.Y., Helfen, L., Schröck, A., Etard, C., Grabher, C., Rastegar, S., Schlunck, G., Reinhard, T., Baumbach, T., Strähle, U. (2016) Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development. Scientific Reports. 6:25046
Wittmann, C., Reischl, M., Shah, A.H., Kronfuss, E., Mikut, R., Liebel, U., Grabher, C. (2015) A Zebrafish Drug-Repurposing Screen Reveals sGC-Dependent and sGC-Independent Pro-Inflammatory Activities of Nitric Oxide. PLoS One. 10:e0137286
Deveau, A.P., Forrester, A.M., Coombs, A.J., Wagner, G.S., Grabher, C., Chute, I.C., Léger, D., Mingay, M., Alexe, G., Rajan, V., Liwski, R., Hirst, M., Steigmaier, K., Lewis, S.M., Look, A.T., Berman, J.N. (2015) Epigenetic therapy restores normal hematopoiesis in a zebrafish model of NUP98-HOXA9-induced myeloid disease. Leukemia. 29(10):2086-97
Wacker, I., Chockley, P., Bartels, C., Spomer, W., Hofmann, A., Gengenbach, U., Singh, S., Thaler, M., Grabher, C., Schröder, R.R. (2015) Array tomography: characterizing FAC-sorted populations of zebrafish immune cells by their 3D ultrastructure. Journal of microscopy. 259(2):105-13
Breckwoldt, M.O., Wittmann, C., Misgeld, T., Kerschensteiner, M., Grabher, C. (2015) Redox imaging using genetically encoded redox indicators in zebrafish and mice. Biological chemistry. 396(5):511-22
Singh, S.K., Aravamudhan, S., Armant, O., Krüger, M., Grabher, C. (2014) Proteome dynamics in neutrophils of adult zebrafish upon chemically-induced inflammation. Fish & shellfish immunology. 40(1):217-24
Singh, S.K., Sethi, S., Aravamudhan, S., Krüger, M., and Grabher, C. (2013) Proteome mapping of adult zebrafish marrow neutrophils reveals partial cross species conservation to human peripheral neutrophils. PLoS One. 8(9):e73998
Bilan, D.S., Pase, L., Joosen, L., Gorokhovatsky, A.Y., Ermakova, Y.G., Gadella, T.W., Grabher, C., Schultz, C., Lukyanov, S., and Belousov, V.V. (2013) HyPer-3: a genetically encoded H2O2 probe with improved performance for ratiometric and fluorescence lifetime imaging. ACS Chemical Biology. 8(3):535-542
Cavodeassi, F., Bene, F.D., Fürthauer, M., Grabher, C., Herzog, W., Lehtonen, S., Linker, C., Mercader, N., Mikut, R., Norton, W., Strähle, U., Tiso, N., and Foulkes, N.S. (2013) Report of the Second European Zebrafish Principal Investigator Meeting in Karlsruhe, Germany, March 21-24, 2012. Zebrafish. 10(1):119-23
Pase, L., Layton, J.E., Wittmann, C., Ellett, F., Nowell, C.J., Reyes-Aldasoro, C.C., Varma, S., Rogers, K.L., Hall, C.J., Keightley, M.C., Crosier, P.S., Grabher, C., Heath, J.K., Renshaw, S.A., and Lieschke, G.J. (2012) Neutrophil-Delivered Myeloperoxidase Dampens the Hydrogen Peroxide Burst after Tissue Wounding in Zebrafish. Current biology : CB. 22(19):1818-1824
Wittmann, C., Reischl, M., Shah, A.H., Mikut, R., Liebel, U., and Grabher, C. (2012) Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish. Journal of visualized experiments : JoVE. (65):e4203
Wittmann, C., Chockley, P., Singh, S.K., Pase, L., Lieschke, G.J., and Grabher, C. (2012) Hydrogen peroxide in inflammation: messenger, guide, and assassin. Advances in hematology. 2012:541471
Forrester, A.M., Grabher, C., McBride, E.R., Boyd, E.R., Vigerstad, M.H., Edgar, A., Kai, F.B., Da'as, S.I., Payne, E., Look, A.T., and Berman, J.N. (2011) NUP98-HOXA9-transgenic zebrafish develop a myeloproliferative neoplasm and provide new insight into mechanisms of myeloid leukaemogenesis. British journal of haematology. 155(2):167-81
Grabher, C., Payne, E.M., Johnston, A.B., Bolli, N., Lechman, E., Dick, J.E., Kanki, J.P., and Look, A.T. (2011) Zebrafish microRNA-126 determines hematopoietic cell fate through c-Myb. Leukemia. 25(3):506-514
d'Alencon, C.A., Pena, O.A., Wittmann, C., Gallardo, V.E., Jones, R.A., Loosli, F., Liebel, U., Grabher, C., and Allende, M.L. (2010) A high-throughput chemically induced inflammation assay in zebrafish. BMC Biology. 8:151
Strähle, U., and Grabher, C. (2010) The zebrafish embryo as a model for assessing off-target drug effects. Disease models & mechanisms. 3(11-12):689-692
Bolli, N., Payne, E.M., Grabher, C., Lee, J.S., Johnston, A.B., Falini, B., Kanki, J.P., and Look, A.T. (2010) Expression of the cytoplasmic NPM1 mutant (NPMc+) causes the expansion of hematopoietic cells in zebrafish. Blood. 115(16):3329-3340
Grabher, C., Patton, E.E., and Strähle, U. (2010) Sharing Chemical Libraries Within the European Zebrafish Community. Zebrafish. 7(1):83
Niethammer, P., Grabher, C., Look, A.T., and Mitchison, T.J. (2009) A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish. Nature. 459(7249):996-999
Zhang, J., Sattler, M., Tonon, G., Grabher, C., Lababidi, S., Zimmerhackl, A., Raab, M.S., Vallet, S., Zhou, Y., Cartron, M.A., Hideshima, T., Tai, Y.T., Chauhan, D., Anderson, K.C., and Podar, K. (2009) Targeting angiogenesis via a c-Myc/hypoxia-inducible factor-1alpha-dependent pathway in multiple myeloma. Cancer research. 69(12):5082-5090
Grabher, C., and Wittbrodt, J. (2008) Recent advances in meganuclease-and transposon-mediated transgenesis of medaka and zebrafish. Methods in molecular biology (Clifton, N.J.). 461:521-539
Kwan, K.M., Fujimoto, E., Grabher, C., Mangum, B.D., Hardy, M.E., Campbell, D.S., Parant, J.M., Yost, H.J., Kanki, J.P., and Chien, C.B. (2007) The Tol2kit: A multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Developmental dynamics : an official publication of the American Association of Anatomists. 236(11):3088-3099
Grabher, C., and Wittbrodt, J. (2007) Meganuclease and transposon mediated transgenesis in medaka. Genome biology. 8(1):S10
Grabher, C., Cliffe, A., Miura, K., Hayflick, J., Pepperkok, R., Rørth, P., and Wittbrodt, J. (2007) Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka. Journal of Leukocyte Biology. 81(1):263-271
Grabher, C., von Boehmer, H., and Look, A.T. (2006) Notch 1 activation in the molecular pathogenesis of T-cell acute lymphoblastic leukaemia. Nature reviews. Cancer. 6(5):347-359
Grabher, C., and Look, A.T. (2006) Fishing for cancer models. Nat. Biotechnol.. 24(1):45-46
Grabher, C., and Wittbrodt, J. (2004) Efficient activation of gene expression using a heat-shock inducible Gal4/Vp16-UAS system in medaka. BMC Biotechnology. 4:26
Grabher, C., Joly, J.S., and Wittbrodt, J. (2004) Highly efficient zebrafish transgenesis mediated by the meganuclease I-SceI. The Zebrafish: Genetics, Genomics and Informatics, 2nd ed., Methods Cell Biol.. 77:381-401
Thermes, V., Grabher, C., Ristoratore, F., Bourrat, F., Choulika, A., Wittbrodt, J., and Joly, J.S. (2002) I-SceI meganuclease mediates highly efficient transgenesis in fish. Mechanisms of Development. 118(1-2):91-98

NON-ZEBRAFISH PUBLICATIONS
Watanabe, T., Asaka, S., Kitagawa, D., Saito, K., Kurashige, R., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Henrich, T., Hirose, Y., Yasuoka, A., Yoda, H., Deguchi, T., Iwanami, N., Kunimatsu, S., Osakada, M., Loosli, F., Quiring, R., Carl, M., Grabher, C., Winkler, S., Del Bene, F., Wittbrodt, J., Abe, K., Takahama, Y., Takahashi, K., Katada, T., Nishina, H., Kondoh, H., and Furutani-Seiki, M. (2004) Mutations affecting liver development and function in Medaka, Oryzias latipes, screened by multiple criteria. Mech. Dev.. 121(7-8):791-802

Loosli, F., Del Bene, F., Quiring, R., Rembold, M., Martinez-Morales, J.R., Carl, M., Grabher, C., Iquel, C., Krone, A., Wittbrodt, B., Winkler, S., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Henrich, T., Deguchi, T., Hirose, Y., Iwanami, N., Kunimatsu, S., Osakada, M., Watanabe, T., Yasuoka, A., Yoda, H., Winkler, C., Elmasri, H., Kondoh, H., Furutani-Seiki, M., and Wittbrodt, J. Mutations affecting retina development in Medaka. Mech Dev. 2004 Jul;121(7-8):703-714. PMID: 15210178.

Kitagawa, D., Watanabe, T., Saito, K., Asaka, S., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Yasuoka, A., Deguchi, T., Yoda, H., Hirose, Y., Henrich, T., Iwanami, N., Kunimatsu, S., Osakada, M., Winkler, C., Elmasri, H., Wittbrodt, J., Loosli, F., Quiring, R., Carl, M., Grabher, C., Winkler, S., Del Bene, F., Momoi, A., Katada, T., Nishina, H., Kondoh, H., and Furutani-Seiki, M. (2004) Genetic dissection of the formation of the forebrain in Medaka, Oryzias latipes. Mech. Dev.. 121(7-8):673-685

Elmasri, H., Winkler, C., Liedtke, D., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Henrich, T., Hirose, Y., Yasuoka, A., Yoda, H., Watanabe, T., Deguchi, T., Iwanami, N., Kunimatsu, S., Osakada, M., Loosli, F., Quiring, R., Carl, M., Grabher, C., Winkler, S., Del Bene, F., Wittbrodt, J., Abe, K., Takahama, Y., Takahashi, K., Katada, T., Nishina, H., Kondoh, H., and Furutani-Seiki, M. (2004) Mutations affecting somite formation in the Medaka (Oryzias latipes). Mech. Dev.. 121(7-8):659-671

Furutani-Seiki, M., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Yoda, H., Deguchi, T., Hirose, Y., Yasuoka, A., Henrich, T., Watanabe, T., Iwanami, N., Kitagawa, D., Saito, K., Asaka, S., Osakada, M., Kunimatsu, S., Momoi, A., Elmasri, H., Winkler, C., Ramialison, M., Loosli, F., Quiring, R., Carl, M., Grabher, C., Winkler, S., Del Bene, F., Shinomiya, A., Kota, Y., Yamanaka, T., Okamoto, Y., Takahashi, K., Todo, T., Abe, K., Takahama, Y., Tanaka, M., Mitani, H., Katada, T., Nishina, H., Nakajima, N., Wittbrodt, J., and Kondoh, H. (2004) A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes. Mech. Dev.. 121(7-8):647-658

Grabher, C., Henrich, T., Sasado, T., Arenz, A., Wittbrodt, J., and Furutani-Seiki, M. Transposon-mediated enhancer trapping in medaka. Gene. 2003 Dec 11;322:57-66. Erratum in: Gene. 2004 Mar 3;327(2):239. PMID: 14644497.

Loosli, F., Winkler, S., Burgtorf, C., Wurmbach, E., Ansorge, W., Henrich, T., Grabher, C., Arendt, D., Carl, M., Krone, A., Grzebisz, E., and Wittbrodt, J. (2001) Medaka eyeless is the key factor linking retinal determination and eye growth. Development. 128(20):4035-4044