Varshney, G.K., Zhang, S., Gildea, D.E., Wolfsberg, T.G., and Burgess, S.M. (2013) The Zebrafish Insertion Collection (ZInC): a web based, searchable collection of zebrafish mutations generated by DNA insertion. Nucleic acids research. 41(D1):D861-864.
ZInC (Zebrafish Insertional Collection, http://research.nhgri.nih.gov/ZInC/) is a web-searchable interface of insertional mutants in zebrafish. Over the last two decades, the zebrafish has become a
popular model organism for studying vertebrate development as well as for modeling human diseases. To facilitate such studies,
we are generating a genome-wide knockout resource that targets every zebrafish protein-coding gene. We developed a streamlined
pipeline utilizing retroviral-mediated insertional mutagenesis together with high-throughput sequencing and mapping technologies.
This is an ongoing project and at present, we have mapped 15 223 unique insertions from 6144 mutagenized F1 fish, generating 7896 integrations in genes with 3776 predicted mutations based on integration site. We will continue to
generate mutations for several more years until the approach is saturated. All mutant fish are freely available to the scientific
community through the Zebrafish International Resource Center (ZIRC). To assist researchers in finding mutant and insertion
information, we developed a comprehensive database with a web front-end, the ZInC. It can be queried using multiple types
of input such as ZFIN (Zebrafish Information Network) IDs, UniGene accession numbers and gene symbols from zebrafish, human
and mouse. In the future, ZInC may include data from other insertional mutation projects as well. ZInC cross-references all
integration data with the ZFIN (ZFIN.org).