ZFIN ID: ZDB-PUB-121205-58
The Zebrafish Insertion Collection (ZInC): a web based, searchable collection of zebrafish mutations generated by DNA insertion
Varshney, G.K., Zhang, S., Gildea, D.E., Wolfsberg, T.G., and Burgess, S.M.
Date: 2013
Source: Nucleic acids research   41(D1): D861-864 (Journal)
Registered Authors: Burgess, Shawn
Keywords: none
MeSH Terms:
  • Animals
  • Databases, Genetic*
  • Gene Knockout Techniques
  • Internet
  • Mutagenesis, Insertional*
  • Mutation
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed: 23180778 Full text @ Nucleic Acids Res.
ABSTRACT

ZInC (Zebrafish Insertional Collection, http://research.nhgri.nih.gov/ZInC/) is a web-searchable interface of insertional mutants in zebrafish. Over the last two decades, the zebrafish has become a popular model organism for studying vertebrate development as well as for modeling human diseases. To facilitate such studies, we are generating a genome-wide knockout resource that targets every zebrafish protein-coding gene. We developed a streamlined pipeline utilizing retroviral-mediated insertional mutagenesis together with high-throughput sequencing and mapping technologies. This is an ongoing project and at present, we have mapped 15 223 unique insertions from 6144 mutagenized F1 fish, generating 7896 integrations in genes with 3776 predicted mutations based on integration site. We will continue to generate mutations for several more years until the approach is saturated. All mutant fish are freely available to the scientific community through the Zebrafish International Resource Center (ZIRC). To assist researchers in finding mutant and insertion information, we developed a comprehensive database with a web front-end, the ZInC. It can be queried using multiple types of input such as ZFIN (Zebrafish Information Network) IDs, UniGene accession numbers and gene symbols from zebrafish, human and mouse. In the future, ZInC may include data from other insertional mutation projects as well. ZInC cross-references all integration data with the ZFIN (ZFIN.org).

ADDITIONAL INFORMATION