Characterization of Ca(2+) signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development
- Authors
- Yuen, M.Y., Webb, S.E., Chan, C.M., Thisse, B., Thisse, C., and Miller, A.L.
- ID
- ZDB-PUB-121130-4
- Date
- 2013
- Source
- Biochimica et biophysica acta. Molecular cell research 1833(7): 1641-56 (Journal)
- Registered Authors
- Miller, Andrew L., Thisse, Bernard, Thisse, Christine, Webb, Sarah E.
- Keywords
- Ca2 + wave, blastula/gastrula period, nuclei clustering, perinuclear endoplasmic reticulum, yolk syncytial layer, zebrafish
- MeSH Terms
-
- Aequorin/metabolism
- Animals
- Blastula/metabolism*
- Calcium Channels/metabolism
- Calcium Signaling/physiology*
- Cell Nucleus/metabolism
- Egg Yolk/cytology
- Egg Yolk/metabolism*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism*
- Endoplasmic Reticulum/metabolism
- Endoplasmic Reticulum/ultrastructure
- Gastrula/metabolism*
- Inositol 1,4,5-Trisphosphate Receptors/metabolism
- Microscopy, Confocal
- Microscopy, Fluorescence, Multiphoton
- Signal Transduction
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism
- PubMed
- 23142640 Full text @ BBA Molecular Cell Research
Preferential loading of the complementary bioluminescent (f-aequorin) and fluorescent (Calcium Green-1 dextran) Ca2 + reporters into the yolk syncytial layer (YSL) of zebrafish embryos, revealed the generation of stochastic patterns of fast, short-range, and slow, long-range Ca2 + waves that propagate exclusively through the external YSL (E-YSL). Starting abruptly just after doming (~ 4.5 h post-fertilization: hpf), and ending at the shield stage (~ 6.0 hpf) these distinct classes of waves propagated at mean velocities of ~ 50 and ~ 4 μm/s, respectively. Although the number and pattern of these waves varied between embryos, their initiation site and arcs of propagation displayed a distinct dorsal bias, suggesting an association with the formation and maintenance of the nascent dorsal-ventral axis. Wave initiation coincided with a characteristic clustering of YSL nuclei (YSN), and their associated perinuclear ER, in the E-YSL. Furthermore, the inter-YSN distance (IND) appeared to be critical such that Ca2 + wave propagation occurred only when this was <~ 8 μm; an IND >~ 8 μm was coincidental with wave termination at shield stage. Treatment with the IP3R antagonist, 2-APB, the Ca2 + buffer, 5,52-dibromo BAPTA, and the SERCA-pump inhibitor, thapsigargin, resulted in a significant disruption of the E-YSL Ca2 + waves, whereas exposure to the RyR antagonists, ryanodine and dantrolene, had no significant effect. These findings led us to propose that the E-YSL Ca2 + waves are generated mainly via Ca2 + release from IP3Rs located in the perinuclear ER, and that the clustering of the YSN is an essential step in providing a CICR pathway required for wave propagation. This article is part of a Special Issue entitled: 12th European Symposium on Calcium.