ZFIN ID: ZDB-PUB-120503-7
An optimized two-finger archive for ZFN-mediated gene targeting
Gupta, A., Christensen, R.G., Rayla, A.L., Lakshmanan, A., Stormo, G.D., and Wolfe, S.A.
Date: 2012
Source: Nature Methods   9(6): 588-590 (Journal)
Registered Authors: Gupta, Ankit, Lakshmanan, Abirami, Wolfe, Scot A.
Keywords: none
MeSH Terms:
  • Animals
  • Catalytic Domain
  • DNA Breaks, Double-Stranded
  • Endodeoxyribonucleases/genetics
  • Endodeoxyribonucleases/metabolism
  • Gene Targeting/methods*
  • Zebrafish
  • Zinc Fingers/genetics*
PubMed: 22543349 Full text @ Nat. Methods

The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.