ZFIN ID: ZDB-PUB-120301-6
Functional analysis of slow Myosin heavy chain 1 and myomesin-3 in sarcomere organization in zebrafish embryonic slow muscles
Xu, J., Gao, J., Li, J., Xue, L., Clark, K.J., Ekker, S.C., and Du, S.J.
Date: 2012
Source: Journal of Genetic Genomics 39(2): 69-80 (Journal)
Registered Authors: Clark, Karl, Du, Shao Jun (Jim), Ekker, Stephen C., Xu, Jin
Keywords: Myosin, Myomesin-3, M-line, sarcomere
MeSH Terms: Animals; Animals, Genetically Modified; In Situ Hybridization; Muscle Proteins/genetics; Muscle Proteins/metabolism* (all 12) expand
PubMed: 22361506 Full text @ J. Genet. Genomics
FIGURES   (current status)
ABSTRACT

Myofibrillogenesis, the process of sarcomere formation, requires close interactions of sarcomeric proteins and various components of sarcomere structures. The myosin thick filaments and M-lines are two key components of the sarcomere. It has been suggested that myomesin proteins of M-lines interact with myosin and titin proteins and keep the thick and titin filaments in order. However, the function of myomesin in myofibrillogenesis and sarcomere organization remained largely enigmatic. No knockout or knockdown animal models have been reported to elucidate the role of myomesin in sarcomere organization in vivo. In this study, by using the gene-specific knockdown approach in zebrafish embryos, we carried out a loss-of-function analysis of myomesin-3 and slow myosin heavy chain 1 (smyhc1) expressed specifically in slow muscles. We demonstrated that knockdown of smyhc1 abolished the sarcomeric localization of myomesin-3 in slow muscles. In contrast, loss of myomesin-3 had no effect on the sarcomeric organization of thick and thin filaments as well as M- and Z-line structures. Together, these studies indicate that myosin thick filaments are required for M-line organization and M-line localization of myomesin-3. In contrast, myomesin-3 is dispensable for sarcomere organization in slow muscles.

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