ZFIN ID: ZDB-PUB-120216-3
Transmission, Diagnosis, and Recommendations for Control of Pseudoloma neurophilia Infections in Laboratory Zebrafish (Danio rerio) Facilities
Murray, K.N., Dreska, M., Nasiadka, A., Rinne, M., Matthews, J.L., Carmichael, C., Bauer, J., Varga, Z.M., and Westerfield, M.
Date: 2011
Source: Comparative medicine   61(4): 322-329 (Journal)
Registered Authors: Bauer, Justin, Carmichael, Carrie, Matthews, Jennifer, Murray, Katy, Nasiadka, Andrzej, Varga, Zoltan M., Westerfield, Monte
Keywords: none
MeSH Terms:
  • Animal Husbandry/methods*
  • Animals
  • Animals, Laboratory*
  • DNA Primers/genetics
  • Fish Diseases/diagnosis*
  • Fish Diseases/microbiology*
  • Fish Diseases/prevention & control
  • Fish Diseases/transmission
  • Histological Techniques/veterinary
  • Microsporidia/genetics*
  • Microsporidiosis/diagnosis
  • Microsporidiosis/prevention & control
  • Microsporidiosis/transmission
  • Microsporidiosis/veterinary*
  • Polymerase Chain Reaction/methods
  • Polymerase Chain Reaction/veterinary
  • Zebrafish*
PubMed: 22330247

The microsporidium Pseudoloma neurophilia represents a considerable challenge for laboratory zebrafish (Danio rerio) facilities. In 2010, P. neurophilia infections were diagnosed in zebrafish from 74% of the facilities that submitted fish to the Zebrafish International Resource Center (ZIRC) pathology service, and this organism remains the most commonly diagnosed pathogen in submitted fish. Accordingly, many of the ZIRC pathology service consultations deal with control and prevention of microsporidiosis. Here we describe observations and experiments performed at the ZIRC elucidating aspects of P. neurophilia transmission in zebrafish colonies. We then review current knowledge about P. neurophilia transmission and diagnosis. Considering this information, we present recommendations for control of P. neurophilia in zebrafish facilities.