PUBLICATION

Fish and Chips: a microfluidic perfusion platform for monitoring zebrafish development

Authors
Choudhury, D., van Noort, D., Iliescu, C., Zheng, B., Poon, K.L., Korzh, S., Korzh, V., and Yu, H.
ID
ZDB-PUB-120105-2
Date
2012
Source
Lab on a Chip   12(5): 892-900 (Journal)
Registered Authors
Korzh, Svitlana, Poon, Kar Lai
Keywords
none
MeSH Terms
  • Animals
  • Microfluidic Analytical Techniques/methods*
  • Perfusion
  • Reproducibility of Results
  • Valproic Acid/pharmacology*
  • Zebrafish/embryology
  • Zebrafish/growth & development*
PubMed
22146879 Full text @ Lab Chip
Abstract

We have developed a multi-channel microfluidic perfusion platform for culturing zebrafish embryos and capturing live images of various tissues and organs inside the embryo. The Fish and Chips was micro-fabricated in silicon and glass for reproducibility and accuracy of the microfluidic structure. The microfluidic platform consists of three parts: a microfluidic gradient generator, a row of eight fish tanks, in which the fish embryos are individually placed, and eight output channels. The fluidic gradient generator supports dose-dependent drug and chemical studies. A unique perfusion system ensures a uniform and constant flow of media to the fish tank while the wastes are efficiently removed. The fish tanks restrict the embryo movements, except rotationally, for live imaging of internal tissues and organs. The embryos showed developmental abnormalities under the influence of the drug valproic acid (VPA).

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes