PUBLICATION

Genetic Screens for Mutations Affecting Adult Traits and Parental-effect Genes

Authors
Pelegri, F., and Mullins, M.C.
ID
ZDB-PUB-110921-7
Date
2011
Source
Methods in cell biology   104: 83-120 (Chapter)
Registered Authors
Mullins, Mary C., Pelegri, Francisco
Keywords
forward genetics, zebrafish, larval, juvenile, adult, fertility, maternal effect, paternal effect, F3-extended family screen, gynogenesis, genetic mapping
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Chromosome Mapping/methods
  • DNA Mutational Analysis/methods*
  • Ethylnitrosourea/pharmacology
  • Female
  • Genes, Recessive
  • Genetic Association Studies/methods*
  • Genetic Testing/methods*
  • Hybridization, Genetic/genetics
  • Larva/genetics
  • Larva/growth & development
  • Male
  • Morphogenesis/genetics
  • Mutagenesis/drug effects
  • Mutagens/pharmacology
  • Phenotype
  • Zebrafish/genetics*
  • Zebrafish/growth & development
PubMed
21924158 Full text @ Meth. Cell. Biol.
Abstract
Forward genetic analysis in the zebrafish has largely until now been restricted to the developmental period from the time of zygotic genome activation through the end of embryogenesis. However, the use of the zebrafish as a model system for the analysis of larval, juvenile and adult traits, including fertility and maternal and paternal effects, is gaining momentum. Here, we describe two different approaches, an F3-extended family and a gynogenesis-based approach, that allow genetic screening for and recovery of mutations affecting post-embryonic stages, including adult traits, fertility, and parental effects. For each approach, we also describe strategies to maintain and map the identified mutations.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping