PUBLICATION
A TALE of Two Nucleases: Gene Targeting for the Masses
- Authors
- Clark, K.J., Voytas, D.F., and Ekker, S.C.
- ID
- ZDB-PUB-110921-32
- Date
- 2011
- Source
- Zebrafish 8(3): 147-149 (Review)
- Registered Authors
- Clark, Karl, Ekker, Stephen C.
- Keywords
- none
- MeSH Terms
-
- Animals
- Deoxyribonucleases/metabolism*
- Gene Targeting*
- Genomics/methods*
- Mutation/genetics
- Zebrafish/genetics
- PubMed
- 21929364 Full text @ Zebrafish
Citation
Clark, K.J., Voytas, D.F., and Ekker, S.C. (2011) A TALE of Two Nucleases: Gene Targeting for the Masses. Zebrafish. 8(3):147-149.
Abstract
Genome editing appears poised to enter an exciting new era. Targeted double-stranded breaks due to custom restriction enzymes are powerful nucleating events for the induction of local changes in the genome. The zinc finger nuclease (ZFN) platform established the potential of this approach for the zebrafish, but access to high quality reagents has been a major bottleneck for the field. However, two groups recently report successful somatic and germline gene modification using a new nuclease architecture, transcription activator-like effector nucleases (TALENs). TALEN construction is simpler, potentially more reliable, and in the few cases examined, shows fewer off-target effects than corresponding ZFNs. TALENs promise to bring gene targeting to the majority of zebrafish laboratories.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping