ZFIN ID: ZDB-PUB-110921-15
Single nucleotide polymorphism (SNP) panels for rapid positional cloning in zebrafish
Clark, M.D., Guryev, V., Bruijn, E., Nijman, I.J., Tada, M., Wilson, C., Deloukas, P., Postlethwait, J.H., Cuppen, E., and Stemple, D.L.
Date: 2011
Source: Methods in cell biology   104: 219-235 (Chapter)
Registered Authors: Clark, Matthew D., Cuppen, Edwin, Guryev, Victor, Postlethwait, John H., Stemple, Derek L., Tada, Masazumi, Wilson, Catherine
Keywords: zebrafish, strains, mapping, mutant, SNP, genotyping, MIPs, KASPar
MeSH Terms:
  • Animals
  • Base Sequence
  • Chromosome Mapping/methods*
  • Cloning, Molecular
  • DNA/genetics
  • DNA/isolation & purification
  • DNA Probes/genetics
  • Genetic Testing/methods
  • Genome
  • Genotype
  • Larva
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • Polymorphism, Single Nucleotide*
  • Sequence Analysis, DNA
  • Zebrafish/genetics*
PubMed: 21924166 Full text @ Meth. Cell. Biol.
Despite considerable genetic and genomic resources the positional cloning of forward mutations remains a slow and manually intensive task, typically using gel based genotyping and sequential rounds of mapping. We have used the latest genetic resources and genotyping technologies to develop two commercially available SNP panels of thousands of markers that can be used to speed up positional cloning.