header logo image header logo text
Downloads Login
General Information
ZFIN ID: ZDB-PUB-110811-21
Targeted gene disruption in somatic zebrafish cells using engineered TALENs
Sander, J.D., Cade, L., Khayter, C., Reyon, D., Peterson, R.T., Joung, J.K., and Yeh, J.R.
Date: 2011
Source: Nat. Biotechnol.   29(8): 697-698 (Journal)
Registered Authors: Peterson, Randall, Yeh, Jing-Ruey (Joanna)
Keywords: none
MeSH Terms:
  • Combinatorial Chemistry Techniques/methods*
  • Genetic Engineering*
  • Humans
  • Mutagenesis, Site-Directed/methods*
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism*
PubMed: 21822241 Full text @ Nat. Biotechnol.
To the Editor: Miller et al.1 recently described a transcription activator–like effector nuclease (TALEN) architecture for efficient genome editing in cultured human cells. We sought to determine whether the same framework could be used to efficiently disrupt endogenous genes in somatic cells of zebrafish and how the efficiency of TALENs compares with that obtained using engineered zinc-finger nucleases (ZFNs).