PUBLICATION

Zebrafish embryos as an alternative to animal experiments-A commentary on the definition of the onset of protected life stages in animal welfare regulations

Authors
Strähle, U., Scholz, S., Geisler, R., Greiner, P., Hollert, H., Rastegar, S., Schumacher, A., Selderslaghs, I., Weiss, C., Witters, H., and Braunbeck, T.
ID
ZDB-PUB-110713-15
Date
2012
Source
Reproductive toxicology (Elmsford, N.Y.)   33(2): 128-132 (Journal)
Registered Authors
Braunbeck, Thomas, Geisler, Robert, Rastegar, Sepand, Strähle, Uwe, Witters, Hilda
Keywords
zebrafish, (eleuthero)embryo, external feeding, protection, alternative method
MeSH Terms
  • Animal Use Alternatives
  • Animal Welfare/legislation & jurisprudence*
  • Animals
  • Embryo, Nonmammalian
  • Feeding Behavior
  • Life Cycle Stages*
  • Social Control, Formal
  • Zebrafish/physiology*
PubMed
21726626 Full text @ Reprod. Toxicol.
Abstract
Worldwide, the zebrafish has become a popular model for biomedical research and (eco)toxicology. Particularly the use of embryos is receiving increasing attention, since they are considered as replacement method for animal experiments. Zebrafish embryos allow the analysis of multiple endpoints ranging from acute and developmental toxicity determination to complex functional genetic and physiological analysis. Particularly the more complex endpoints require the use of post-hatched eleutheroembryo stages. According to the new EU Directive 2010/63/EU on the protection of animals used for scientific purposes, the earliest life-stages of animals are not defined as protected and, therefore, do not fall into the regulatory frameworks dealing with animal experimentation. Independent feeding is considered as the stage from which free-living larvae are subject to regulations for animal experimentation. However, despite this seemingly clear definition, large variations exist in the interpretation of this criterion by national and regional authorities. Since some assays require the use of post-hatched stages up to 120 h post fertilization, the literature and available data are reviewed in order to evaluate if this stage could still be considered as non-protected according to the regulatory criterion of independent feeding. Based on our analysis and by including criteria such as yolk consumption, feeding and swimming behavior, we conclude that zebrafish larvae can indeed be regarded as independently feeding from 120 h after fertilization. Experiments with zebrafish should thus be subject to regulations for animal experiments from 120 h after fertilization onwards.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping