The knockdown of maternal glucocorticoid receptor mRNA alters embryo development in zebrafish
- Pikulkaew, S., Benato, F., Celeghin, A., Zucal, C., Skobo, T., Colombo, L., and Dalla Valle , L.
- Developmental dynamics : an official publication of the American Association of Anatomists 240(4): 874-889 (Journal)
- Registered Authors
- Benato, Francesca, Dalla Valle, Luisa
- zebrafish, gr mRNA, morpholino (MO), development
- MeSH Terms
- Animals, Genetically Modified
- Base Sequence
- Embryo, Nonmammalian
- Embryonic Development/genetics*
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- Larva/growth & development
- Molecular Sequence Data
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- RNA, Messenger, Stored/genetics*
- RNA, Messenger, Stored/metabolism
- Receptors, Glucocorticoid/genetics*
- Receptors, Glucocorticoid/metabolism
- 21360790 Full text @ Dev. Dyn.
Pikulkaew, S., Benato, F., Celeghin, A., Zucal, C., Skobo, T., Colombo, L., and Dalla Valle , L. (2011) The knockdown of maternal glucocorticoid receptor mRNA alters embryo development in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 240(4):874-889.
In zebrafish, ovulated oocytes contain both maternal cortisol and the mRNA for the glucocorticoid receptor (gr), which is spread as granular structures throughout the ooplasm. At 0.2 hpf, this transcript is relocated in the blastodisc area and partitioned among blastomeres. At 6-8 hpf, it is replaced by zygotic transcript. We used morpholinos to block translation of both maternal and zygotic gr transcripts, and a missplicing morpholino to block post-transcriptionally the zygotic transcript alone. Only knockdown of translation produced an increase of apoptosis and subsequent craniofacial and caudal deformities with severe malformations of neural, vascular, and visceral organs in embryos and 5-dpf larvae. Such defects were rescued with trout gr2 mRNA. Microarray analysis revealed that 114 and 37 highly expressed transcripts were up- and down-regulated, respectively, by maternal Gr protein deficiency in 5-hpf embryos. These results indicate that the maternal gr transcript and protein participate in the maternal programming of zebrafish development.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes