PUBLICATION

A dual role for ErbB2 signaling in cardiac trabeculation

Authors
Liu, J., Bressan, M., Hassel, D., Huisken, J., Staudt, D., Kikuchi, K., Poss, K.D., Mikawa, T., and Stainier, D.Y.
ID
ZDB-PUB-101101-3
Date
2010
Source
Development (Cambridge, England)   137(22): 3867-3875 (Journal)
Registered Authors
Hassel, David, Huisken, Jan, Kikuchi, Kazu, Liu, Jiandong, Poss, Kenneth D., Stainier, Didier, Staudt, David
Keywords
Cardiac trabeculation, Endocardium, SPIM, ErbB2, Zebrafish
MeSH Terms
  • Animals
  • Cell Movement
  • Cell Proliferation
  • Heart/embryology*
  • Morphogenesis*
  • Myocardium/metabolism
  • Myocytes, Cardiac/metabolism*
  • Receptor, ErbB-2/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
PubMed
20978078 Full text @ Development
Abstract
Cardiac trabeculation is a crucial morphogenetic process by which clusters of ventricular cardiomyocytes extrude and expand into the cardiac jelly to form sheet-like projections. Although it has been suggested that cardiac trabeculae enhance cardiac contractility and intra-ventricular conduction, their exact function in heart development has not been directly addressed. We found that in zebrafish erbb2 mutants, which we show completely lack cardiac trabeculae, cardiac function is significantly compromised, with mutant hearts exhibiting decreased fractional shortening and an immature conduction pattern. To begin to elucidate the cellular mechanisms of ErbB2 function in cardiac trabeculation, we analyzed erbb2 mutant hearts more closely and found that loss of ErbB2 activity resulted in a complete absence of cardiomyocyte proliferation during trabeculation stages. In addition, based on data obtained from proliferation, lineage tracing and transplantation studies, we propose that cardiac trabeculation is initiated by directional cardiomyocyte migration rather than oriented cell division, and that ErbB2 cell-autonomously regulates this process.
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Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes