ZFIN ID: ZDB-PUB-101027-6
Characterization of voltage-activated ionic currents in the GnRH-containing terminalis nerve in transgenic zebrafish
Huang, L., and Li, L.
Date: 2011
Source: Brain research   1367: 43-49 (Journal)
Registered Authors: Li, Lei
Keywords: zebrafish, transgene, voltage-activated current, terminalis nerve, olfactory bulb
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Biophysical Phenomena/drug effects
  • Biophysical Phenomena/physiology*
  • Cells, Cultured
  • Electric Stimulation/methods
  • Gonadotropin-Releasing Hormone/genetics
  • Gonadotropin-Releasing Hormone/metabolism*
  • Green Fluorescent Proteins/genetics
  • Ion Channel Gating/drug effects
  • Ion Channel Gating/physiology
  • Ion Channels/classification
  • Ion Channels/drug effects
  • Ion Channels/physiology*
  • Membrane Potentials/drug effects
  • Membrane Potentials/physiology*
  • Membrane Transport Modulators/pharmacology
  • Neurons/cytology*
  • Olfactory Bulb/cytology
  • Patch-Clamp Techniques/methods
  • Presynaptic Terminals/physiology*
  • Zebrafish
PubMed: 20951681 Full text @ Brain Res.
The terminalis nerve (TN) is in a class of cranial nerves that plays important roles in animal development, physiology and behavior. Here, we report a study on the characterization of voltage-activated ionic currents in GnRH-containing TN cells in zebrafish. The experiments were performed using acutely dissociated TN cells from the transgenic zebrafish Tg (GnRH-3::GFP). In the transgenic zebrafish, the TN cells express GFP under the transcriptional control of the zebrafish GnRH-3 promoter. In all of the GnRH-containing TN cells examined, we recorded both low-voltage activated (LVA) and high-voltage activated (HVA) calcium current (I(Ca)). The characteristics of the I(Ca) were similar to those described in other zebrafish cell types. However, the distribution patterns of the currents in the GnRH-containing TN cells were different in comparison to the distribution of the currents in other cell types. In addition, we characterized TTX-sensitive sodium current (I(Na)) and 4AP-sensitive and TEA-resistant potassium current (I(K)). The characteristics of voltage-activated I(Na) and I(K) in the GnRH-containing TN cells were similar to those described in other zebrafish cell types. Together, the data from this study revealed the electrophysiological properties of the GnRH-containing TN cells, thereby providing insight on the regulatory mechanisms of TN-signaling in animal physiology.