ZFIN ID: ZDB-PUB-100702-2
Intraflagellar Transport Proteins Are Essential for Cilia Formation and for Planar Cell Polarity
Cao, Y., Park, A., and Sun, Z.
Date: 2010
Source: Journal of the American Society of Nephrology : JASN   21(8): 1326-1333 (Journal)
Registered Authors: Cao, Ying, Park, Alice, Sun, Zhaoxia
Keywords: none
MeSH Terms:
  • Adaptor Proteins, Signal Transducing/genetics*
  • Animals
  • Cell Polarity/genetics*
  • Cilia/genetics*
  • Cilia/physiology
  • Mutation
  • Zebrafish
  • Zebrafish Proteins/genetics*
PubMed: 20576807 Full text @ J. Am. Soc. Nephrol.
ABSTRACT
The highly conserved intraflagellar transport (IFT) proteins are essential for cilia formation in multiple organisms, but surprisingly, cilia form in multiple zebrafish ift mutants. Here, we detected maternal deposition of ift gene products in zebrafish and found that ciliary assembly occurs only during early developmental stages, supporting the idea that maternal contribution of ift gene products masks the function of IFT proteins during initial development. In addition, the basal bodies in multiciliated cells of the pronephric duct in ift mutants were disorganized, with a pattern suggestive of defective planar cell polarity (PCP). Depletion of pk1, a core PCP component, similarly led to kidney cyst formation and basal body disorganization. Furthermore, we found that multiple ift genes genetically interact with pk1. Taken together, these data suggest that IFT proteins play a conserved role in cilia formation and planar cell polarity in zebrafish.
ADDITIONAL INFORMATION