PUBLICATION
Zebrafish as a Model for the Study of the Phase II Cytosolic Sulfotransferases
- Authors
- Liu, T.A., Bhuiyan, S., Liu, M.Y., Sugahara, T., Sakakibara, Y., Suiko, M., Yasuda, S., Kakuta, Y., Kimura, M., Williams, F.E., and Liu, M.C.
- ID
- ZDB-PUB-100621-9
- Date
- 2010
- Source
- Current drug metabolism 11(6): 538-546 (Review)
- Registered Authors
- Williams, Fred
- Keywords
- Cytosolic sulfotransferase, SULT, Sulfation, Phase II enzyme, Zebrafish, Danio Rerio, Drug metabolism
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cytosol/enzymology*
- Humans
- Models, Animal*
- Molecular Sequence Data
- Multigene Family
- Sulfotransferases/biosynthesis
- Sulfotransferases/genetics
- Sulfotransferases/metabolism*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 20545621 Full text @ Curr. Drug Metab.
Citation
Liu, T.A., Bhuiyan, S., Liu, M.Y., Sugahara, T., Sakakibara, Y., Suiko, M., Yasuda, S., Kakuta, Y., Kimura, M., Williams, F.E., and Liu, M.C. (2010) Zebrafish as a Model for the Study of the Phase II Cytosolic Sulfotransferases. Current drug metabolism. 11(6):538-546.
Abstract
Cytosolic sulfotransferases (SULTs) are traditionally known as the Phase II drug-metabolizing or detoxifying enzymes that serve for the detoxification of drugs and other xenobiotics. These enzymes in general catalyze the transfer of a sulfonate group from the active sulfate, 3'-phophoadenosine 5'-phosphosulfate (PAPS), to low-molecular weight substrate compounds containing hydroxyl or amino group(s). Despite considerable efforts made in recent years, some fundamental aspects of the SULTs, particularly their ontogeny, cell type/tissue/organ-specific distribution, and physiological relevance, particularly their involvement in drug metabolism and detoxification, still remain poorly understood. To better understand these fundamental issues, we have embarked on developing the zebrafish as a model for studies concerning the SULTs. To date, fifteen zebrafish SULTs have been cloned, expressed, purified, and characterized. These zebrafish SULTs, which fall into four major SULT gene families, exhibited differential substrate specificities and distinct patterns of expression at different stages during embryogenesis, through larval development, and on to maturity. The information obtained, as summarized in this review, provides a foundation for further investigation into the physiological and pharmacological involvement of the SULTs using the zebrafish as a model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping