PUBLICATION

Small Molecule Screen in Zebrafish and HSC Expansion

Authors
Trompouki, E., and Zon, L.I.
ID
ZDB-PUB-100330-23
Date
2010
Source
Methods in molecular biology (Clifton, N.J.)   636: 301-316 (Chapter)
Registered Authors
Zon, Leonard I.
Keywords
Zebrafish hematopoiesis, HSCs: hematopoietic stem cells, Chemical screen, Chemical compound library, Chemoinformatics
MeSH Terms
  • Animals
  • Computational Biology/methods
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/physiology*
  • Female
  • Hematopoiesis/physiology
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/physiology*
  • Humans
  • Male
  • Signal Transduction/physiology
  • Small Molecule Libraries*
  • Zebrafish*
PubMed
20336531 Full text @ Meth. Mol. Biol.
Abstract
The zebrafish (Danio rerio) has emerged as a valuable model organism that is amenable for large-scale chemical and genetic screens. The ability of zebrafish to produce large quantities of synchronized, externally fertilized, transparent embryos makes them ideal for screens, which often are not possible in mammalian models. Signaling pathways important for hematopoiesis are well conserved between zebrafish and mammals, making many targets identified in zebrafish screens applicable to mammals. Hematopoiesis in zebrafish occurs in two waves: the primitive or embryonic wave and the definitive or adult wave. Definitive hematopoietic stem cells arise in the aorta-gonad-mesonephros region (AGM) and express conserved markers such as runx1 and c-myb that allow for the detection of stem cells by whole-mount in situ hybridization (WISH). In this protocol, we will discuss a chemical screen in zebrafish embryos to detect compounds that expand or deplete hematopoietic stem cells (HSCs) in vivo. This type of screen represents a powerful tool to study HSCs in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping