|ZFIN ID: ZDB-PUB-100105-11|
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Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells
Kajita, M., Hogan, C., Harris, A.R., Dupre-Crochet, S., Itasaki, N., Kawakami, K., Charras, G., Tada, M., and Fujita, Y.
|Source:||Journal of Cell Science 123(Pt 2): 171-180 (Journal)|
|Registered Authors:||Kawakami, Koichi, Tada, Masazumi|
|Keywords:||Apical extrusion, Focal adhesion kinase, Mitogen-activated protein kinase, Myosin-II, Src, Zebrafish|
|PubMed:||20026643 Full text @ J. Cell Sci.|
Kajita, M., Hogan, C., Harris, A.R., Dupre-Crochet, S., Itasaki, N., Kawakami, K., Charras, G., Tada, M., and Fujita, Y. (2010) Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells. Journal of Cell Science. 123(Pt 2):171-180.
ABSTRACTAt the initial stage of carcinogenesis, transformation occurs in a single cell within an epithelial sheet. However, it remains unknown what happens at the boundary between normal and transformed cells. Using Madin-Darby canine kidney (MDCK cells transformed with temperature-sensitive v-Src, we have examined the interface between normal and Src-transformed epithelial cells. We show that Src-transformed cells are apically extruded when surrounded by normal cells, but not when Src cells alone are cultured, suggesting that apical extrusion occurs in a cell-context-dependent manner. We also observe apical extrusion of Src-transformed cells in the enveloping layer of zebrafish gastrula embryos. When Src-transformed MDCK cells are surrounded by normal MDCK cells, myosin-II and focal adhesion kinase (FAK are activated in Src cells, which further activate downstream mitogen-activated protein kinase (MAPK. Importantly, activation of these signalling pathways depends on the presence of surrounding normal cells and plays a crucial role in apical extrusion of Src cells. Collectively, these results indicate that interaction with surrounding normal epithelial cells influences the signalling pathways and behaviour of Src-transformed cells.