ZFIN ID: ZDB-PUB-100105-11
Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells
Kajita, M., Hogan, C., Harris, A.R., Dupre-Crochet, S., Itasaki, N., Kawakami, K., Charras, G., Tada, M., and Fujita, Y.
Date: 2010
Source: Journal of Cell Science   123(Pt 2): 171-180 (Journal)
Registered Authors: Kawakami, Koichi, Tada, Masazumi
Keywords: Apical extrusion, Focal adhesion kinase, Mitogen-activated protein kinase, Myosin-II, Src, Zebrafish
MeSH Terms:
  • Animals
  • Cadherins/metabolism
  • Cell Adhesion
  • Cell Communication*
  • Cell Polarity
  • Cell Transformation, Neoplastic/metabolism*
  • Cell Transformation, Neoplastic/pathology*
  • Dogs
  • Epithelial Cells/cytology*
  • Epithelial Cells/metabolism*
  • Focal Adhesion Protein-Tyrosine Kinases/metabolism
  • Humans
  • Mitogen-Activated Protein Kinases/metabolism
  • Models, Biological
  • Myosin Type II/metabolism
  • Oncogene Protein pp60(v-src)/metabolism*
  • Protein Transport
  • Signal Transduction*
  • Zebrafish/metabolism
  • beta Catenin/metabolism
PubMed: 20026643 Full text @ J. Cell Sci.
At the initial stage of carcinogenesis, transformation occurs in a single cell within an epithelial sheet. However, it remains unknown what happens at the boundary between normal and transformed cells. Using Madin-Darby canine kidney (MDCK cells transformed with temperature-sensitive v-Src, we have examined the interface between normal and Src-transformed epithelial cells. We show that Src-transformed cells are apically extruded when surrounded by normal cells, but not when Src cells alone are cultured, suggesting that apical extrusion occurs in a cell-context-dependent manner. We also observe apical extrusion of Src-transformed cells in the enveloping layer of zebrafish gastrula embryos. When Src-transformed MDCK cells are surrounded by normal MDCK cells, myosin-II and focal adhesion kinase (FAK are activated in Src cells, which further activate downstream mitogen-activated protein kinase (MAPK. Importantly, activation of these signalling pathways depends on the presence of surrounding normal cells and plays a crucial role in apical extrusion of Src cells. Collectively, these results indicate that interaction with surrounding normal epithelial cells influences the signalling pathways and behaviour of Src-transformed cells.