PUBLICATION
The zebrafish prospero homolog prox1 is required for mechanosensory hair cell differentiation and functionality in the lateral line
- Authors
- Pistocchi, A., Feijoo, C.G., Cabrera, P., Villablanca, E.J., Allende, M.L., and Cotelli, F.
- ID
- ZDB-PUB-091215-19
- Date
- 2009
- Source
- BMC Developmental Biology 9: 58 (Journal)
- Registered Authors
- Allende, Miguel L., Cabrera, Pablo, Cotelli, Franco, Feijoo, Carmen G.
- Keywords
- none
- MeSH Terms
-
- Mechanoreceptors/cytology
- Mechanoreceptors/metabolism*
- Cell Differentiation
- Homeodomain Proteins
- Tumor Suppressor Proteins
- Animals
- Embryo, Nonmammalian/metabolism
- Lateral Line System/cytology
- Lateral Line System/embryology*
- Lateral Line System/metabolism
- Gene Expression Regulation, Developmental
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 19948062 Full text @ BMC Dev. Biol.
Citation
Pistocchi, A., Feijoo, C.G., Cabrera, P., Villablanca, E.J., Allende, M.L., and Cotelli, F. (2009) The zebrafish prospero homolog prox1 is required for mechanosensory hair cell differentiation and functionality in the lateral line. BMC Developmental Biology. 9:58.
Abstract
BACKGROUND: The lateral line system in zebrafish is composed of a series of organs called neuromasts, which are distributed over the body surface. Neuromasts contain clusters of hair cells, surrounded by accessory cells.
RESULTS: In this report we describe zebrafish prox1 mRNA expression in the migrating primordium and in the neuromasts of the posterior lateral line. Furthermore, using an antibody against Prox1 we characterize expression of the protein in different cell types within neuromasts, and we show distribution among the supporting cells and hair cells.
CONCLUSION: Functional analysis using antisense morpholinos indicates that prox1 activity is crucial for the hair cells to differentiate properly and acquire functionality, while having no role in development of other cell types in neuromasts.
RESULTS: In this report we describe zebrafish prox1 mRNA expression in the migrating primordium and in the neuromasts of the posterior lateral line. Furthermore, using an antibody against Prox1 we characterize expression of the protein in different cell types within neuromasts, and we show distribution among the supporting cells and hair cells.
CONCLUSION: Functional analysis using antisense morpholinos indicates that prox1 activity is crucial for the hair cells to differentiate properly and acquire functionality, while having no role in development of other cell types in neuromasts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping