PUBLICATION
Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka
- Authors
- Grabher, C., Cliffe, A., Miura, K., Hayflick, J., Pepperkok, R., Rørth, P., and Wittbrodt, J.
- ID
- ZDB-PUB-091204-2
- Date
- 2007
- Source
- Journal of Leukocyte Biology 81(1): 263-271 (Journal)
- Registered Authors
- Grabher, Clemens
- Keywords
- Macrophage, cell migration, medaka, PI3K, inflammation
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Chemotaxis*
- Embryonic Development/physiology*
- Inflammation/metabolism*
- Leukocytes/metabolism
- Macrophages/physiology*
- Oryzias/immunology*
- Phosphatidylinositol 3-Kinases/metabolism
- Signal Transduction
- PubMed
- 17046968 Full text @ J. Leukoc. Biol.
Citation
Grabher, C., Cliffe, A., Miura, K., Hayflick, J., Pepperkok, R., Rørth, P., and Wittbrodt, J. (2007) Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka. Journal of Leukocyte Biology. 81(1):263-271.
Abstract
Macrophages detecting and migrating toward sites of injury and infection represent one of the first steps in an immune response. Here we directly image macrophage birth and migration in vivo in transgenic medaka fish. Macrophages are born as frequently dividing, immotile cells with spherical morphology that differentiate into flat, highly motile cells. They retain mitotic activity while spreading over the entire body. Cells follow restricted paths not only in directed migration, but also during patrolling. Along those paths the macrophages rapidly patrol the tissue and respond to wounding and bacterial infection from long distances. Upon injury they increase their speed and migratory persistence. Specifically targeting PI3-kinase isoforms efficiently blocks the wounding response and results in a distinct inhibition of cell motility and chemotaxis. Our study provides in situ insights into the properties of immature and migratory macrophages and presents a unique model to further test modulating compounds in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping