PUBLICATION

Differential role for synaptojanin 1 in rod and cone photoreceptors

Authors
Holzhausen, L.C., Lewis, A.A., Cheong, K.K., and Brockerhoff, S.E.
ID
ZDB-PUB-091023-42
Date
2009
Source
The Journal of comparative neurology   517(5): 633-644 (Journal)
Registered Authors
Brockerhoff, Susan, Lewis, Alaron
Keywords
synaptojanin, zebrafish, photoreceptors, ribbon synapse, endocytosis
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Larva/anatomy & histology
  • Larva/metabolism
  • Mutation
  • Phosphoric Monoester Hydrolases/deficiency
  • Phosphoric Monoester Hydrolases/metabolism*
  • Retinal Cone Photoreceptor Cells/cytology
  • Retinal Cone Photoreceptor Cells/metabolism*
  • Retinal Rod Photoreceptor Cells/cytology
  • Retinal Rod Photoreceptor Cells/metabolism*
  • Synapses/metabolism*
  • Zebrafish/anatomy & histology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/metabolism*
PubMed
19827152 Full text @ J. Comp. Neurol.
Abstract
Synaptojanin 1 (SynJ1) is a polyphosphoinositide phosphatase involved in clathrin-mediated endocytosis in conventional synapses. Studies with the zebrafish mutant nrc have revealed that loss of SynJ1 also affects cone photoreceptor ribbon synapses, causing pronounced morphological and functional abnormalities. In this study we continue to examine the role of SynJ1 in photoreceptors. Using a newly generated antibody specific for zebrafish SynJ1, we localized this protein predominantly to cone photoreceptors. We then used blastula stage transplantation experiments to demonstrate that rods from nrc mutants lacking SynJ1 develop normally and do not have the pronounced morphological defects detected in cones. Given the known involvement of SynJ1 in synaptic vesicle endocytosis, we hypothesize that rods and cones use distinct mechanisms for vesicle recycling.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping