ZFIN ID: ZDB-PUB-090914-38
N-CoR is required for patterning the anterior-posterior axis of zebrafish hindbrain by actively repressing retinoid signaling
Xu, F., Li, K., Tian, M., Hu, P., Song, W., Chen, J., Gao, X., and Zhao, Q.
Date: 2009
Source: Mechanisms of Development   126(10): 771-780 (Journal)
Registered Authors: Hu, Ping, Song, Wei, Xu, Fang, Zhao, Qingshun
Keywords: Zebrafish, N-CoR, Retinoid signaling, Hindbrain, Anterior-posterior axis, Rhombomere, Cyp26A1, Aldh1a2, Hox
MeSH Terms:
  • Animals
  • Base Sequence
  • Body Patterning/physiology*
  • Cloning, Molecular
  • Co-Repressor Proteins/genetics
  • Co-Repressor Proteins/physiology*
  • DNA, Complementary
  • Gene Knockdown Techniques
  • In Situ Hybridization
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Rhombencephalon/embryology*
  • Signal Transduction/physiology*
  • Tretinoin/metabolism*
  • Zebrafish/embryology*
PubMed: 19735730 Full text @ Mech. Dev.
Active repression of gene expression mediated by unliganded nuclear receptors plays crucial roles in early development of vertebrate. N-CoR (nuclear receptor co-repressor) is the first identified co-repressor that can repress retinoic acid (RA) inducible gene transcription in the absence of RA. Previously, N-CoR was reported to be required for late-stage organogenesis in mouse but whether N-CoR can affect RA-responsive early embryonic patterning is unknown. In this study, we report molecular cloning of zebrafish orthologue of N-CoR and its wide distribution pattern during zebrafish early development. Knocking down n-cor elevates endogenous RA signaling in zebrafish embryos and posteriorizes the neural ectoderm. Overexpressing or knocking down n-cor in zebrafish embryos alters the length of hindbrain in a manner similar to decreasing or increasing RA signaling in embryos respectively. Taken together, our results demonstrate that N-CoR is essential for early hindbrain patterning by actively repressing retinoid signaling.