PUBLICATION

The two groups of zebrafish virus-induced interferons signal via distinct receptors with specific and shared chains

Authors
Aggad, D., Mazel, M., Boudinot, P., Mogensen, K.E., Hamming, O.J., Hartmann, R., Kotenko, S., Herbomel, P., Lutfalla, G., and Levraud, J.P.
ID
ZDB-PUB-090914-12
Date
2009
Source
Journal of immunology (Baltimore, Md. : 1950)   183(6): 3924-3931 (Journal)
Registered Authors
Herbomel, Philippe, Levraud, Jean-Pierre, Lutfalla, Georges
Keywords
none
MeSH Terms
  • Animals
  • Chromosomes
  • Conserved Sequence
  • Cysteine
  • Cytokines
  • Gene Components
  • Interferons/genetics
  • Interferons/metabolism*
  • Protein Structure, Quaternary
  • Receptors, Interferon/chemistry
  • Receptors, Interferon/genetics
  • Receptors, Interferon/metabolism*
  • Signal Transduction/immunology*
  • Viruses/immunology*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
19717522 Full text @ J. Immunol.
Abstract
Because the availability of fish genomic data, the number of reported sequences for fish type II helical cytokines is rapidly growing, featuring different IFNs including virus-induced IFNs (IFNphi) and IFN-gamma, and IL-10 with its related cytokines (IL-20, IL-22, and IL-26). Many candidate receptors exist for these cytokines and various authors have postulated which receptor chain would be involved in which functional receptor in fish. To date, only the receptor for zebrafish IFNphi1 has been identified functionally. Three genes encoding virus-induced IFNphis have been reported in zebrafish. In addition to these genes clustered on chromosome 3, we have identified a fourth IFNphi gene on chromosome 12. All these genes possess the intron-exon organization of mammalian lambda IFNs. In the zebrafish larva, all induce the expression of reporter antiviral genes; protection in a viral challenge assay was observed for IFNphi1 and IFNphi2. Using a combination of gain- and loss-of-function experiments, we also show that all zebrafish IFNphis do not bind to the same receptor. Two subgroups of fish virus-induced IFNs have been defined based on conserved cysteines, and we find that this subdivision correlates with receptor usage. Both receptor complexes include a common short chain receptor (CRFB5) and a specific long chain receptor (CRFB1 or CRFB2).
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes