PUBLICATION

Hematopoietic stem cell development is dependent on blood flow

Authors
North, T.E., Goessling, W., Peeters, M., Li, P., Ceol, C., Lord, A.M., Weber, G.J., Harris, J., Cutting, C.C., Huang, P., Dzierzak, E., and Zon, L.I.
ID
ZDB-PUB-090526-18
Date
2009
Source
Cell   137(4): 736-748 (Journal)
Registered Authors
Ceol, Craig, Goessling, Wolfram, Lord, Allegra, North, Trista, Weber, Gerhard, Zon, Leonard I.
Keywords
DEVBIO, STEMCELL
MeSH Terms
  • Animals
  • Blood Physiological Phenomena*
  • Embryo, Mammalian/metabolism
  • Embryo, Nonmammalian/metabolism
  • Hematopoiesis*
  • Hematopoietic Stem Cells/cytology*
  • Mice
  • Mice, Inbred C57BL
  • Nitric Oxide/metabolism
  • Nitric Oxide Synthase Type III/metabolism
  • Zebrafish
PubMed
19450519 Full text @ Cell
Abstract
During vertebrate embryogenesis, hematopoietic stem cells (HSCs) arise in the aorta-gonads-mesonephros (AGM) region. We report here that blood flow is a conserved regulator of HSC formation. In zebrafish, chemical blood flow modulators regulated HSC development, and silent heart (sih) embryos, lacking a heartbeat and blood circulation, exhibited severely reduced HSCs. Flow-modifying compounds primarily affected HSC induction after the onset of heartbeat; however, nitric oxide (NO) donors regulated HSC number even when treatment occurred before the initiation of circulation, and rescued HSCs in sih mutants. Morpholino knockdown of nos1 (nnos/enos) blocked HSC development, and its requirement was shown to be cell autonomous. In the mouse, Nos3 (eNos) was expressed in HSCs in the AGM. Intrauterine Nos inhibition or embryonic Nos3 deficiency resulted in a reduction of hematopoietic clusters and transplantable murine HSCs. This work links blood flow to AGM hematopoiesis and identifies NO as a conserved downstream regulator of HSC development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes