ZFIN ID: ZDB-PUB-090511-2
FlEx-based transgenic reporter lines for visualization of Cre and Flp activity in live zebrafish
Boniface, E.J., Lu, J., Victoroff, T., Zhu, M., and Chen, W.
Date: 2009
Source: Genesis (New York, N.Y. : 2000) 47(7): 484-491 (Journal)
Registered Authors: Chen, Wenbiao
Keywords: Cre, Flp, CreERT2, reporter line, enhancer trap, fluorescent proteins, tissue-specific Cre, conditional Cre, zebrafish
MeSH Terms: Animals; Animals, Genetically Modified; Autophagy*; Base Sequence; DNA Nucleotidyltransferases/genetics (all 12) expand
PubMed: 19415631 Full text @ Genesis
ABSTRACT
Site-specific recombinases such as Cre and Flp are invaluable tools for genetic manipulations, but their usage in zebrafish has been limited. Incorporating recently developed flip-excision (FlEx) design that allows stable inversions, we have established zebrafish reporter lines that express bright and ubiquitous EGFP, but switch to express mCherry in the presence of Cre or Flp. Here, we demonstrate the stable inversion in the reporter lines, both in somatic cells and in the germ line by Cre or Flp, and the subsequent reinversion using the other recombinase. Using the reporter lines, we characterized cardiomyocyte-specific Cre lines and neuronal progenitor-specific and tamoxifen-dependent Cre lines. We also used the reporter lines for screening Cre- and Flp-based enhancer trap lines. Similar to the widely used Cre reporter lines in mice, these FlEx-based reporter lines will facilitate the use of recombinases for genetic manipulations in zebrafish.
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