PUBLICATION
Morphologic analysis of the zebrafish digestive system
- Authors
- Trotter, A.J., Parslow, A.C., and Heath, J.K.
- ID
- ZDB-PUB-090424-11
- Date
- 2009
- Source
- Methods in molecular biology (Clifton, N.J.) 546: 289-315 (Chapter)
- Registered Authors
- Heath, Joan K., Parslow, Adam, Trotter, Andrew
- Keywords
- Zebrafish, Intestine, Epithelium, Goblet cells, Enteroendocrine cells, Histology, Immunohistochemistry, Transgenic lines
- MeSH Terms
-
- Microscopy/instrumentation
- Microscopy/methods
- Recombinant Fusion Proteins/biosynthesis
- Recombinant Fusion Proteins/genetics
- Organogenesis
- Green Fluorescent Proteins
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Immunohistochemistry/instrumentation
- Immunohistochemistry/methods
- Digestive System/cytology
- Digestive System/embryology*
- Digestive System/metabolism
- Genes, Reporter
- Animals
- Image Processing, Computer-Assisted/instrumentation
- Image Processing, Computer-Assisted/methods
- PubMed
- 19378111 Full text @ Meth. Mol. Biol.
Citation
Trotter, A.J., Parslow, A.C., and Heath, J.K. (2009) Morphologic analysis of the zebrafish digestive system. Methods in molecular biology (Clifton, N.J.). 546:289-315.
Abstract
The zebrafish provides an ideal model for the study of vertebrate organogenesis, including the formation of the digestive tract and its associated organs. Despite optical transparency of embryos, the internal position of the developing digestive system and its close juxtaposition with the yolk initially made morphological analysis relatively challenging, particularly during the first 3 d of development. However, methodologies have been successfully developed to address these problems and comprehensive morphologic analysis of the developing digestive system has now been achieved using a combination of light and fluorescence microscope approaches-including confocal analysis-to visualize wholemount and histological preparations of zebrafish embryos. Furthermore, the expanding number of antibodies that cross-react with zebrafish proteins and the generation of tissue-specific transgenic green fluorescent protein reporter lines that mark specific cell and tissue compartments have greatly enhanced our ability to successfully image the developing zebrafish digestive system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping