ZFIN ID: ZDB-PUB-090422-16
A primer for morpholino use in zebrafish
Bill, B.R., Petzold, A.M., Clark, K.J., Schimmenti, L.A., and Ekker, S.C.
Date: 2009
Source: Zebrafish 6(1): 69-77 (Review)
Registered Authors: Bill, Brent, Clark, Karl, Ekker, Stephen C., Petzold, Andrew
Keywords: none
MeSH Terms:
  • Animals
  • Gene Knockdown Techniques*
  • Oligonucleotides/genetics*
  • Zebrafish/genetics*
PubMed: 19374550 Full text @ Zebrafish
Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.