PUBLICATION

Quantum dot nanotoxicity assessment using the zebrafish embryo

Authors
King-Heiden, T.C., Wiecinski, P.N., Mangham, A.N., Metz, K.M., Nesbit, D., Pedersen, J.A., Hamers, R.J., Heideman, W., and Peterson, R.E.
ID
ZDB-PUB-090413-3
Date
2009
Source
Environmental science & technology   43(5): 1605-1611 (Journal)
Registered Authors
Heideman, Warren, Peterson, Richard E.
Keywords
none
MeSH Terms
  • Animals
  • Cadmium/metabolism
  • Cadmium/toxicity
  • Embryo, Nonmammalian/drug effects*
  • Larva/drug effects
  • Metallothionein/metabolism
  • Nanostructures/toxicity*
  • Polylysine/metabolism
  • Quantum Dots*
  • Survival Analysis
  • Suspensions
  • Time Factors
  • Toxicity Tests*
  • Zebrafish/embryology*
PubMed
19350942 Full text @ Env. Sci. Tech.
Abstract
Quantum dots (QDs) hold promise for several biomedical, life sciences, and photovoltaic applications. Substantial production volumes and environmental release are anticipated. QD toxicity may be intrinsic to their physicochemical properties, or result from the release of toxic components during breakdown. We hypothesized that developing zebrafish could be used to identify and distinguish these different types of toxicity. Embryos were exposed to aqueous suspensions of CdSe(core)/ZnS(shell) QDs functionalized with either poly-L-lysine or poly(ethylene glycol) terminated with methoxy, carboxylate, or amine groups. Toxicity was influenced by the QD coating, which also contributed to the QD suspension stability. At sublethal concentrations, many QD preparations produced characteristic signs of Cd toxicity that weakly correlated with metallothionein expression, indicating that QDs are only slightly degraded in vivo. QDs also produced distinctly different toxicity that could not be explained by Cd release. Using the zebrafish model, we were able to distinguish toxicity intrinsic to QDs from that caused by released metal ions. We conclude that developing zebrafish provide a rapid, low-cost approach for assessing structure-toxicity relationships of nanoparticles.
Genes / Markers
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes