PUBLICATION

NF-kappaB and Snail1a coordinate the cell cycle with gastrulation

Authors
Liu, X., Huang, S., Ma, J., Li, C., Zhang, Y., and Luo, L.
ID
ZDB-PUB-090330-4
Date
2009
Source
The Journal of cell biology   184(6): 805-815 (Journal)
Registered Authors
Luo, Lingfei, Ma, Jun
Keywords
none
MeSH Terms
  • Animals
  • Apoptosis
  • Blastula/metabolism*
  • Cell Cycle*/genetics
  • Cell Cycle Proteins/metabolism
  • Cell Movement
  • Cell Nucleus/metabolism
  • Cytoplasm/metabolism
  • Gastrula/metabolism*
  • Gastrulation*/genetics
  • Gene Expression Regulation, Developmental
  • Nervous System/embryology
  • Nervous System/metabolism
  • Promoter Regions, Genetic
  • RNA, Messenger/metabolism
  • Signal Transduction*/genetics
  • Transcription Factor RelA/genetics
  • Transcription Factor RelA/metabolism*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Transcriptional Activation
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
19307597 Full text @ J. Cell Biol.
Abstract
The cell cycle needs to strictly coordinate with developmental processes to ensure correct generation of the body plan and different tissues. However, the molecular mechanism underlying the coordination remains largely unknown. In this study, we investigate how the cell cycle coordinates gastrulation cell movements in zebrafish. We present a system to modulate the cell cycle in early zebrafish embryos by manipulating the geminin-Cdt1 balance. Alterations of the cell cycle change the apoptotic level during gastrulation, which correlates with the nuclear level of antiapoptotic nuclear factor kappaB (NF-kappaB). NF-kappaB associates with the Snail1a promoter region on the chromatin and directly activates Snail1a, an important factor controlling cell delamination, which is the initial step of mesendodermal cell movements during gastrulation. In effect, the cell cycle coordinates the delamination of mesendodermal cells through the transcription of Snail1a. Our results suggest a molecular mechanism by which NF-kappaB and Snail1a coordinate the cell cycle through gastrulation.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes