PUBLICATION
Temporally-controlled site-specific recombination in zebrafish
- Authors
- Hans, S., Kaslin, J., Freudenreich, D., and Brand, M.
- ID
- ZDB-PUB-090302-32
- Date
- 2009
- Source
- PLoS One 4(2): e4640 (Journal)
- Registered Authors
- Brand, Michael, Freudenreich, Dorian, Hans, Stefan, Kaslin, Jan
- Keywords
- Embryos, Zebrafish, Alleles, Recombinant proteins, In situ hybridization, Diencephalon, Gene mapping, Plasmid construction
- MeSH Terms
-
- Recombination, Genetic*
- Zebrafish/genetics*
- In Situ Hybridization
- Immunohistochemistry
- Animals
- PubMed
- 19247481 Full text @ PLoS One
Citation
Hans, S., Kaslin, J., Freudenreich, D., and Brand, M. (2009) Temporally-controlled site-specific recombination in zebrafish. PLoS One. 4(2):e4640.
Abstract
Conventional use of the site-specific recombinase Cre is a powerful technology in mouse, but almost absent in other vertebrate model organisms. In zebrafish, Cre-mediated recombination efficiency was previously very low. Here we show that using transposon-mediated transgenesis, Cre is in fact highly efficient in this organism. Furthermore, temporal control of recombination can be achieved by using the ligand-inducible CreER(T2). Site-specific recombination only occurs upon administration of the drug tamoxifen (TAM) or its active metabolite, 4-hydroxy-tamoxifen (4-OHT). Cre-mediated recombination is detectable already 4 or 2 hours after administration of TAM or 4-OHT, demonstrating fast recombination kinetics. In addition, low doses of TAM allow mosaic labeling of single cells. Combined, our results show that conditional Cre/lox will be a valuable tool for both, embryonic and adult zebrafish studies. Furthermore, single copy insertion transgenesis of Cre/lox constructs suggest a strategy suitable also for other organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping